首页> 外文期刊>Chembiochem: A European journal of chemical biology >Methane Hydroxylation with Water as an Electron Donor under Light Irradiation in the Presence of Reconstituted Membranes Containing both Photosystem?II and a Methane Monooxygenase

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Methane Hydroxylation with Water as an Electron Donor under Light Irradiation in the Presence of Reconstituted Membranes Containing both Photosystem?II and a Methane Monooxygenase

机译：在含有含有光系统αII和甲烷单氧化酶的重构膜存在下，用水作为电子供体的甲烷羟基。

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Abstract > Methane/methanol conversion is one of the most important chemical reactions. Methane monooxygenases from methanotrophs are enzymes that catalyze methane/methanol conversion under mild conditions. Here we report the reconstitution of purified photosystem?II (PSII) from Thermosynechococcus elongatus BP‐1 into the membrane fraction containing particulate methane monooxygenase (pMMO) from Methylosinus trichosporium OB3b. Photoinduced hydroxylation of methane to methanol was successfully achieved by using the PSII‐reconstituted membrane containing pMMO under light irradiation. This result indicates that the sequential redox chain from PSII through the quinone pool to pMMO can be constructed and that water can serve as the electron donor for methane hydroxylation under irradiation with light. pMMO in the membrane fraction produced hydrogen peroxide as a byproduct when an electron donor was added for methane hydroxylation, whereas under light irradiation conditions the PSII‐reconstituted membrane containing pMMO did not generate hydrogen peroxide. Optimization of the electron‐transfer rate can easily be achieved with this system by tuning the light intensity. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“main”XML：Lang =“en”> <标题类型=“main”>摘要</标题> >甲烷/甲醇转化是最重要的化学反应之一。来自甲基丙醇的甲烷单氧基酶是在温和条件下催化甲烷/甲醇转化率的酶。在这里，我们将纯化的光系统αi（psii）从热梭聚合物的重建重构，进入含有颗粒甲烷单氧基酶（PMMO）的膜馏分（PMMO），从甲基骨肉滴孢子酸钙-1 ob3b。通过在光照射下使用PSII-重构的PMMO，成功地实现了甲烷的甲烷至甲醇的羟基化。该结果表明，可以构建来自PSII通过醌池到PMMO的顺序氧化还原链，并且水可以用作甲烷羟基的电子供体，用光照射。当向甲烷羟基加入电子给体时，在膜馏分中产生的PMMO产生过氧化氢作为副产物，而在光照射条件下，含有含有过氧化氢的PSII-重构的膜没有产生过氧化氢。通过调整光强度，可以通过该系统轻松实现电子传输速率的优化。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-16039/'>《Chembiochem: A European journal of chemical biology》</a> <b style="margin: 0 2px;">|</b><span>2018年第20期</span><b style="margin: 0 2px;">|</b><span>共4页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ito Hidehiro&option=202" target="_blank" rel="nofollow">Ito Hidehiro;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kondo Ryuichi&option=202" target="_blank" rel="nofollow">Kondo Ryuichi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yoshimori Kosei&option=202" target="_blank" rel="nofollow">Yoshimori Kosei;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kamachi Toshiaki&option=202" target="_blank" rel="nofollow">Kamachi Toshiaki;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Life Science and TechnologyTokyo Institute of Technology2-12-1-M6-7 Ookayama Meguro-ku Tokyo 152-8550 Japan;</p> <p>Department of Life Science and TechnologyTokyo Institute of Technology2-12-1-M6-7 Ookayama Meguro-ku Tokyo 152-8550 Japan;</p> <p>Department of Life Science and TechnologyTokyo Institute of Technology2-12-1-M6-7 Ookayama Meguro-ku Tokyo 152-8550 Japan;</p> <p>Department of Life Science and TechnologyTokyo Institute of Technology2-12-1-M6-7 Ookayama Meguro-ku Tokyo 152-8550 Japan;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/179.html" title="分子生物学">分子生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=hydroxylation&option=203" rel="nofollow">hydroxylation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=methane hydroxylation&option=203" rel="nofollow">methane hydroxylation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=methane monooxygenases&option=203" rel="nofollow">methane monooxygenases;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=photosystem?II&option=203" rel="nofollow">photosystem?II;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=membranes&option=203" rel="nofollow">membranes;</a> </p> <div class="translation"> 机译：羟基化;甲烷羟基化;甲烷单氧基酶;光系统？II;膜; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022056360.html">Methane Hydroxylation with Water as an Electron Donor under Light Irradiation in the Presence of Reconstituted Membranes Containing both Photosystem?II and a Methane Monooxygenase</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ito Hidehiro&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ito 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<span>机译：制备氟碘甲烷，包括使二碘甲烷与碘五氟和/或三氟溴化反应在胺多氟化氢化合物的存在下，任选地在催化剂的存在下进行。氧化铜 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130451791117.html">Process for lightening the color of polymeric diphenylmethane diisocyanate and the use of brightened diphenylmethane diisocyanate in the production of polyurethane plastics</a> <b>[P]</b> . <span> 外国专利：  DE19815055A1 </span> <span> . 1999-10-07</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：淡化聚合的二苯基甲烷二异氰酸酯的颜色的方法以及增亮的二苯基甲烷二异氰酸酯在聚氨酯塑料生产中的用途 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130449872808.html">Process for lightening the color of polymeric diphenylmethane diisocyanate and the use of brightened diphenylmethane diisocyanate in the production of polyurethane</a> <b>[P]</b> . <span> 外国专利：  US5994579A </span> <span> . 1999-11-30</span> 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