Enhanced Cleavage at Abasic Sites within Clustered Lesions in Nucleosome Core Particles

机译：在核小体核心颗粒中聚集病变内的脱脂位点的增强裂解

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Abstract >Clustered lesions are a hallmark of γ‐radiolysis, but are produced by other damaging agents as well. Bistranded clustered lesions are precursors to double‐strand breaks and are challenging to repair, thus making them an especially deleterious form of DNA damage. An abasic site (AP) is an alkaline‐labile lesion frequently present in clustered lesions. Strand scission at an AP site is accelerated ≈100‐fold in nucleosome core particles (NCPs). We examined how AP reactivity was affected within clustered lesions in NCPs. The rate constant of strand scission is increased as much as 2.5‐fold in the presence of a proximal abasic site or thymidine glycol in the complementary strand. A proximal mispair has a similar effect on AP reactivity. Increased AP reactivity within a clustered lesion correlates with decreased UV melting temperatures of the corresponding duplexes compared to one containing an isolated abasic site. However, the thermodynamics of duplex melting do not correlate with AP reactivity within different clustered lesions. Overall, increased AP reactivity within clustered lesions is attributed to greater access of histone proteins to the lesion due to decreased duplex stability. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >聚类病变是γ-辐射溶解的标志，但也由其他损坏的药剂产生。 Bistranded聚类病变是双链休息的前体，并挑战修复，从而使它们成为一种特别有害的DNA损伤形式。 Abasic Site（AP）是常存在聚类病变中的碱性不稳定病变。 AP位点的股线易于在核心核心颗粒（NCPS）中加速≈100倍。我们检查了如何在NCPS的聚类病变中影响AP反应性。在互补链中存在近似脱脂位点或胸苷乙二醇的存在下，股线裂变的速率常数增加了2.5倍。近端错误的错误对AP反应性具有类似的效果。与含有孤立的脱脂位点的一个相比，簇损伤内的AP反应性与相应双链体的降低的UV熔化温度相关。然而，双工熔化的热力学与不同聚类病变内的AP反应性不相关。总的来说，簇状病变内的AP反应性增加归因于由于双相稳定性降低，组蛋白蛋白对损伤的更大接近。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-16039/'>《Chembiochem: A European journal of chemical biology》</a> <b style="margin: 0 2px;">|</b><span>2018年第19期</span><b style="margin: 0 2px;">|</b><span>共5页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Kun&option=202" target="_blank" rel="nofollow">Yang Kun;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Greenberg Marc M.&option=202" target="_blank" rel="nofollow">Greenberg Marc M.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of ChemistryJohns Hopkins University3400 N. Charles St. Baltimore MD 21218 USA;</p> <p>Department of ChemistryJohns Hopkins University3400 N. Charles St. Baltimore MD 21218 USA;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/179.html" title="分子生物学">分子生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=DNA damage&option=203" rel="nofollow">DNA damage;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=DNA oxidation&option=203" rel="nofollow">DNA oxidation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=nucleosome core particle&option=203" rel="nofollow">nucleosome core particle;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=gamma-radiolysis&option=203" rel="nofollow">gamma-radiolysis;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=reaction mechanism&option=203" rel="nofollow">reaction mechanism;</a> </p> <div class="translation"> 机译：DNA损伤;DNA氧化;核心核心颗粒;γ-辐射溶解;反应机制; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022055776.html">Enhanced Cleavage at Abasic Sites within Clustered Lesions in Nucleosome Core Particles</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Kun&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yang Kun,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Greenberg Marc M.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Greenberg Marc M. </a> <a href="/journal-foreign-16039/" target="_blank" rel="nofollow" class="tuijian_authcolor">Chembiochem: A European journal of chemical biology .</a> <span>2018</span><span>,第19期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：在核小体核心颗粒中聚集病变内的脱脂位点的增强裂解</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704027903230.html">Rotational Effects within Nucleosome Core Particles on Abasic Site Reactivity</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Ruixiang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wang Ruixiang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Kun&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yang Kun,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Banerjee Samya&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Banerjee Samya,</a> <a href="/journal-foreign-14809/" target="_blank" rel="nofollow" class="tuijian_authcolor">Biochemistry .</a> <span>2018</span><span>,第26期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：核心核心颗粒中的旋转效应在Abasic位点反应性</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704021725848.html">Repair efficiency of clustered abasic sites by APE1 in nucleosome core particles is sequence and position dependent</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Singh Vandana&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Singh Vandana,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kumari Bhavini&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Kumari Bhavini,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Das Prolay&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Das Prolay </a> <a href="/journal-foreign-27299/" target="_blank" rel="nofollow" class="tuijian_authcolor">RSC Advances .</a> <span>2015</span><span>,第30期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过APE1在核心核心颗粒中的聚集脱脂位点的修复效率是序列和位置</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-265148_thesis/0705011973520.html">Fluorescence studies on the conformational changes of nucleosome core particles</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=David W. Brown&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">David W. Brown,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Louis J. Libertini&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Louis J. Libertini,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Enoch W. Small&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Enoch W. Small </a> <a href="/conference-foreign-265148/" target="_blank" rel="nofollow" class="tuijian_authcolor">Fluorescence Science and Technology .</a> <span>2000</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：荧光研究关于核心核心颗粒的构象变化</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/0206182872.html">Computational modeling of DNA sequence effects on the nucleosome core particle.</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pierce, Levi CT.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Pierce, Levi CT. </a> <span>2008</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：DNA序列对核小体核心颗粒影响的计算模型。</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040005234629.html">Enhanced Cleavage at Abasic Sites Within Clustered Lesions in Nucleosome Core Particles</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kun Yang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Kun Yang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Marc M. Greenberg&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Marc M. Greenberg </a> <span>-1</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：增强切割在核小体核心颗粒中的群集病变的基础位点。</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/01000131045352.html">Enhanced Cleavage at Abasic Sites within Clustered Lesions in Nucleosome Core Particles</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kun Yang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Kun Yang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Marc M. Greenberg&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Marc M. Greenberg </a> <span>2018</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：在核小体核心颗粒中聚集病变内的脱脂位点的增强裂解</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_geriatrics-health-care_thesis/0201230841635.html">老年人几种高凝疾患中血小板聚集ATP-释放与血浆内血小板α-颗粒膜蛋白的关系</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王豫廉&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 王豫廉</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=华见&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,华见</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=费云捷&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,费云捷</a> <span> <a href="/journal-cn-5971/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 老年医学与保健 </a> </span> <span> . 1998</span><span>,第001期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201295246024.html">一种聚合物裂解—反应热压制备纳米SiC颗粒增强MoSi2基复合材料的方法</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . </a> <span> <a href="/journal-cn-57332/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 发明与创新：大科技 </a> </span> <span> . 2013</span><span>,第5期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_ciesc-journal_thesis/0201290801011.html">新型催化裂解快速流化床内颗粒浓度分布实验研究</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王景效&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 王景效</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=贺翔宇&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,贺翔宇</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=龚剑洪&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,龚剑洪</a> <span> <a href="/journal-cn-9151/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 化工学报 </a> </span> <span> . 2021</span><span>,第008期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-minimally-invasive-neurosurgery_thesis/0201273521076.html">基于三维重建模型的增强现实技术在颅内病变术前定位中的应用研究</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张威&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 张威</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=常骁毅&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,常骁毅</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=高珂&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,高珂</a> <span> <a href="/journal-cn-6112/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国微侵袭神经外科杂志 </a> </span> <span> . 2019</span><span>,第012期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_china-medical-education-technology_thesis/0201210148305.html">虚拟和增强现实技术在颅内病变穿刺手术教学中的应用</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张家墅&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 张家墅</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=余新光&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,余新光</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=许百男&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,许百男</a> <span> <a href="/journal-cn-2798/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国医学教育技术 </a> </span> <span> . 2017</span><span>,第005期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-223_thesis/02022342795.html">CT动态增强扫描对肝内孤立性结节病变鉴别诊断探讨</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liu Zhaojun&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Liu Zhaojun</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘兆军&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,刘兆军</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liu Liyi&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Liu Liyi</a> <span> <a href="/conference-cn-223/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第十四次中国中西医结合医学影像学术研讨会 </a> <span> <span> . 2016</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020314050446.html">钆增强快速双回波自旋回波序列MRI在颅内强化病变检出中的价值：理论分析、模型实验及初步临床应用研究</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张德生&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 张德生</a> <span> . 2007</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120110525636.html">应用于组织样本中核小体位点MNase酶切优化方法</a> <b>[P]</b> . <span> 中国专利： CN108048543A </span> <span> . 2018-05-18</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120110673799.html">应用于组织样本中核小体位点MNase酶切优化方法</a> <b>[P]</b> . <span> 中国专利： CN108179179A </span> <span> . 2018-06-19</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130409469106.html">rna targeting dna; DNA polynucleotide; recombinant expression vector; genetically modified host cell in vitro; variant site-directed modification polypeptide; chimeric site-directed modification polypeptide; rna polynucleotide; genetically modified cell; transgenic non-human organism whose genome comprises a transgene; composition; site specific modification method of a target DNA; site specific transcriptional modulation method within a target DNA; site-specific modification method in target DNA; method of promoting site-specific cleavage and modification of a target DNA in a cell; method of producing a genetically modified cell in a subject; method of modifying target DNA in a genetically modified cell; kit; target dna target kit; method of selectively modulating transcription of a target DNA into a host cell; isolated nucleic acid; genetically modified host cell; and nucleic acid library</a> <b>[P]</b> . <span> 外国专利：  BR112014029441A2 </span> <span> . 2017-06-27</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：针对dna的rna; DNA多核苷酸;重组表达载体体外转基因宿主细胞;变体定点修饰多肽;嵌合定点修饰多肽; rna多核苷酸;转基因细胞基因组包含转基因的转基因非人类生物;组成;靶DNA的位点特异性修饰方法;靶DNA内的位点特异性转录调节方法;靶DNA的位点特异性修饰方法;促进细胞中靶DNA的位点特异性切割和修饰的方法;在受试者中产生基因修饰的细胞的方法;在转基因细胞中修饰靶DNA的方法;套件目标dna目标试剂盒;选择性调节靶DNA转录到宿主细胞中的方法;分离的核酸转基因宿主细胞;和核酸文库 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130435435632.html">Method for the characterization of nucleic acid molecules involving generation of extendible upstream DNA fragments resulting from the cleavage of nucleic acid at an abasic site</a> <b>[P]</b> . <span> 外国专利：  US7175982B1 </span> <span> . 2007-02-13</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：鉴定核酸分子的方法，该方法涉及由无碱基位点的核酸切割产生的可延伸的上游DNA片段的产生 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130437536455.html">A METHOD FOR THE CHARACTERISATION OF NUCLEIC ACID MOLECULES INVOLVING GENERATION OF EXTENDIBLE UPSTREAM DNA FRAGMENTS RESULTING FROM THE CLEAVAGE OF NUCLEIC ACID AT AN ABASIC SITE</a> <b>[P]</b> . <span> 外国专利：  CA2325854C </span> <span> . 2005-12-06</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：鉴定涉及在碱性位点裂解核酸而产生的可扩展上游DNA片段的核酸分子的表征方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704022055776','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche">  </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);">回到顶部</a> </li> <li class="shouye"> <a href="/">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html">关于掌桥</a></li> <li><a href="/help/helpmap.html">资源导航</a></li> <li><a href="/help/helpguide.html">新手指南</a></li> <li><a href="/help/helpcenter.html">常见问题</a></li> <li><a href="/sitemap.html">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704022055776down" data-source="7," data-out-id="7fPL/LnA1CY6PuFhmUGMyacDg11Qd2UUCYUubxZWjNf5i3XU0qRM9l499sFdiTF7," data-f-source-id="7" data-title="Enhanced Cleavage at Abasic Sites within Clustered Lesions in Nucleosome Core Particles" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="16039" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="Enhanced Cleavage at Abasic Sites within Clustered Lesions in Nucleosome Core Particles"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.7"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.7"></script> </body> <script> $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>