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首页> 外文期刊>Chembiochem: A European journal of chemical biology >Determination of Alkyl‐Donor Promiscuity of Tyrosine‐ OO ‐Prenyltransferase SirD from Leptosphaeria maculansLeptosphaeria maculans
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Determination of Alkyl‐Donor Promiscuity of Tyrosine‐ OO ‐Prenyltransferase SirD from Leptosphaeria maculansLeptosphaeria maculans

机译：从瘦性的甲基转移酶SiRD的溶磷酸溶磷酸酯溶液的烷基供体滥用的测定 - 瘦磷酸甲基汞 - 乳酸溶核马草

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Abstract > Natural product prenyltransferases are known to display relaxed acceptor substrate specificity. Although recent studies with a small set of unnatural alkyl donors have revealed that prenyltransferases are flexible with regard to their alkyl donors, the scope of their alkyl donor specificity remains poorly understood. Towards this goal, we report the synthesis of 20 unnatural alkyl pyrophosphate donors and an assessment of the reactions of these synthetic unnatural alkyl pyrophosphate analogues catalyzed by tyrosine O ‐prenyltransferase SirD. This study demonstrates that SirD can utilize 16 out of 21 alkyl pyrophosphate analogues (including the natural donor) in catalyzing mostly O‐alkylation of <sc>l</sc> ‐tyrosine. This study reveals the broad alkyl donor specificity of SirD and opens the door for the interrogation of the alkyl donor specificity of other prenyltransferases for potential utility as biocatalysts for differential alkylation applications. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>摘要</标题> >天然产物戊基转移酶显示出弛豫的受体底物特异性。尽管近期具有一小组非自然烷基供体的研究表明，在其烷基供体方面是柔韧的，其烷基供体特异性的范围仍然是较差的。对此目标，我们报道了由酪氨酸催化催化的20个非自然烷基焦磷酸盐供体的合成和评估这些合成的非自然烷基焦磷酸酯类似物的反应。该研究表明，SyRIRD可以利用21种烷基焦磷酸盐类似物（包括天然供体）中的16种，催化<sc> L </ sc> -Titosine的O-烷基化。本研究揭示了克雷的宽烷基供体特异性，并打开了用于鉴别其他戊酰基转移酶的烷基供体特异性的门，以潜在效用作为用于差动烷基化应用的生物催化剂。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-16039/'>《Chembiochem: A European journal of chemical biology》</a> <b style="margin: 0 2px;">|</b><span>2017年第23期</span><b style="margin: 0 2px;">|</b><span>共5页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bandari Chandrasekhar&option=202" target="_blank" rel="nofollow">Bandari Chandrasekhar;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Scull Erin M.&option=202" target="_blank" rel="nofollow">Scull Erin M.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Masterson Johanna M.&option=202" target="_blank" rel="nofollow">Masterson Johanna M.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tran Rachel H. Q.&option=202" target="_blank" rel="nofollow">Tran Rachel H. Q.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Foster Steven B.&option=202" target="_blank" rel="nofollow">Foster Steven B.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nicholas Kenneth M.&option=202" target="_blank" rel="nofollow">Nicholas Kenneth M.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Singh Shanteri&option=202" target="_blank" rel="nofollow">Singh Shanteri;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Institute for Natural Products Applications and Research TechnologiesUniversity of Oklahoma101 Stephenson Parkway Norman Oklahoma 73019 USA;</p> <p>Institute for Natural Products Applications and Research TechnologiesUniversity of Oklahoma101 Stephenson Parkway Norman Oklahoma 73019 USA;</p> <p>Institute for Natural Products Applications and Research TechnologiesUniversity of Oklahoma101 Stephenson Parkway Norman Oklahoma 73019 USA;</p> <p>Institute for Natural Products Applications and Research TechnologiesUniversity of Oklahoma101 Stephenson Parkway Norman Oklahoma 73019 USA;</p> <p>Institute for Natural Products Applications and Research TechnologiesUniversity of Oklahoma101 Stephenson Parkway Norman Oklahoma 73019 USA;</p> <p>Institute for Natural Products Applications and Research TechnologiesUniversity of Oklahoma101 Stephenson Parkway Norman Oklahoma 73019 USA;</p> <p>Institute for Natural Products Applications and Research TechnologiesUniversity of Oklahoma101 Stephenson Parkway Norman Oklahoma 73019 USA;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/179.html" title="分子生物学">分子生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=alkylation&option=203" rel="nofollow">alkylation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=natural products&option=203" rel="nofollow">natural products;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=prenyltransferases&option=203" rel="nofollow">prenyltransferases;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=sirodesmin&option=203" rel="nofollow">sirodesmin;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=unnatural donors&option=203" rel="nofollow">unnatural donors;</a> </p> <div class="translation"> 机译：烷基化;天然产物;戊烯转移酶;纱线;不自然的捐助者; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022055291.html">Determination of Alkyl‐Donor Promiscuity of Tyrosine‐ <i >OO ‐Prenyltransferase SirD from <i >Leptosphaeria maculansLeptosphaeria maculans</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bandari Chandrasekhar&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Bandari Chandrasekhar,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Scull Erin M.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Scull Erin M.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Masterson Johanna M.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Masterson Johanna M.,</a> <a href="/journal-foreign-16039/" target="_blank" rel="nofollow" class="tuijian_authcolor">Chembiochem: A European journal of chemical biology .</a> <span>2017</span><span>,第23期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：从瘦性的甲基转移酶SiRD的溶磷酸溶磷酸酯溶液的烷基供体滥用的测定 - 瘦磷酸甲基汞 - 乳酸溶核马草</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025775966.html">Distribution of mating‐type alleles and genetic variability in field populations of Leptosphaeria maculansLeptosphaeria maculans in western Canada</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zou Zhongwei&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zou Zhongwei,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Xuehua&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhang Xuehua,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fernando Wannakuwattewaduge Gerard Dilantha&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Fernando Wannakuwattewaduge Gerard Dilantha </a> <a href="/journal-foreign-35479/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of Phytopathology .</a> <span>2018</span><span>,第6期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：加拿大西部百分之育荔枝猕猴桃盐肺粉黄瓜野外群体交配型等位基因及遗传变异的分布</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/070402685446.html">Tyrosine O -prenyltransferase SirD catalyzes S -, C -, and N -prenylations on tyrosine and tryptophan derivatives</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rudolf J.D.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Rudolf J.D.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Poulter C.D.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Poulter C.D. </a> <a href="/journal-foreign-18812/" target="_blank" rel="nofollow" class="tuijian_authcolor">ACS Chemical Biology .</a> <span>2013</span><span>,第12期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：酪氨酸O-异戊烯基转移酶SirD催化酪氨酸和色氨酸衍生物的S-，C-和N-异戊二烯化</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040005690595.html">The Tyrosine O-Prenyltransferase SirD Catalyzes S- C- and N-Prenylations on Tyrosine and Tryptophan Derivatives</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jeffrey D. 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