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Acetylation is the most abundant actin modification in Entamoeba histolytica Entamoeba histolyticaEntamoeba histolytica and modifications of actin's amino‐terminal domain change cytoskeleton activities

机译：乙酰化是 entamoeba组织olytica中最丰富的肌动蛋白修饰术中最丰富的肌动蛋白修饰术语 entamoeba组织olytica entamoeba组织olytica和actin的氨基末端结构域的修饰改变细胞骨架活性

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Abstract > Actin is one of the most conserved, abundant, and ubiquitous proteins in all eukaryotes characterised to date. Posttranslation modifications of actin modify the organisation of the actin‐rich cytoskeleton. In particular, chemical modifications of actin's amino‐terminal region determine how filamentous actin is organised into scaffolds. After assuming that protein modifications account for the multiple functional activities exerted by the single actin in <fc> <fi>Entamoeba histolytica</fi> </fc> , we profiled posttranslational modifications of this protein. Acetylation (on 21 different amino acids) was the most abundant modification, followed by phosphorylation. Furthermore, the glycine residue at Position 2 in <fc> <fi>E. histolytica</fi> </fc> 's actin (Gly2, not found in most other eukaryotic actins) was found to be acetylated. The impact of Gly2 on the amoeba's life cycle and pathogenicity was then assessed in mutagenesis experiments. We found that Gly2 was necessary for cell morphology and division, parasite–host cell adhesion, and host invasion in an in vitro model of amoebic human infection. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“En”> <Title Type =“Main”> Abstract </ Title> <P> Actin是所有真核生物中最受保守，丰富和无处不在的蛋白质之一，其特征在于迄今为止。肌动蛋白的后翻译修饰改性富含致动蛋白的细胞骨架的组织。特别地，actin的氨基末端区域的化学修饰决定了如何将丝状肌动蛋白组织成支架。在假设蛋白质修饰占据单个肌动蛋白中的多个功能活动之后，在<FC> <fi> entamoeba组织olytica </ fi> </ fc>中，我们对该蛋白质的发生后改性。乙酰化（在21种不同的氨基酸）是最丰富的修饰，然后是磷酸化。此外，在<Fc> <fi> e的位置2中的甘氨酸残基。发现组神溶剂（在大多数其他真核actins中未发现的gly2，在大多数其他真核actins中）被发现是乙酰化的。然后在诱变实验中评估Gly2对AmoEBA的生命周期和致病性的影响。我们发现Gly2是细胞形态和分区，寄生虫 - 宿主细胞粘附和在amoebic人类感染的体外模型中的宿主侵袭。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15891/'>《Cellular microbiology》</a> <b style="margin: 0 2px;">|</b><span>2019年第4期</span><b style="margin: 0 2px;">|</b><span>共15页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hernández‐Cuevas Nora Adriana&option=202" target="_blank" rel="nofollow">Hernández‐Cuevas Nora Adriana;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jhingan Gagan Deep&option=202" target="_blank" rel="nofollow">Jhingan Gagan Deep;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Petropolis Debora&option=202" target="_blank" rel="nofollow">Petropolis Debora;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Vargas Miguel&option=202" target="_blank" rel="nofollow">Vargas Miguel;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guillen Nancy&option=202" target="_blank" rel="nofollow">Guillen Nancy;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Institut PasteurUnité Biologie Cellulaire du ParasitismeParis France;</p> <p>National Institute of ImmunologyNew Delhi India;</p> <p>Institut PasteurUnité Biologie Cellulaire du ParasitismeParis France;</p> <p>Departamento de Biomedicina MolecularInstituto Politécnico Nacional Centro de Investigación y de Estudios Avanzados CINVESTAVMexico City Mexico;</p> <p>Institut PasteurUnité Biologie Cellulaire du ParasitismeParis France;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/173.html" title="普通生物学">普通生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022032135.html">Acetylation is the most abundant actin 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