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首页> 外文期刊>Cellular microbiology >Proteomics analysis of Medicago truncatula Medicago truncatulaMedicago truncatula response to infection by the phytopathogenic bacterium Ralstonia solanacearum Ralstonia solanacearumRalstonia solanacearum points to jasmonate and salicylate defence pathways
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Proteomics analysis of Medicago truncatula Medicago truncatulaMedicago truncatula response to infection by the phytopathogenic bacterium Ralstonia solanacearum Ralstonia solanacearumRalstonia solanacearum points to jasmonate and salicylate defence pathways

机译： medicago truncatula medicago truncatula medicago truncatula植物感染的蛋白质组学分析 medicago truncatula响应于植物疗法菌株 ralstonia solanacearum < / fc> ralstonia solanacearum Ralstonia solanacearum指向茉莉酸盐和水杨酸盐防御途径

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Abstract > The infection of the model legume <fc> <fi>Medicago truncatula</fi> </fc> with <fi>Ralstonia solanacearum</fi> GMI1000 gives rise to bacterial wilt disease via colonisation of roots. The root and leaf responses to early infection (1 and 3?days post infection) were characterised to investigate the molecular mechanisms of plant resistance or susceptibility. A proteomics approach based on pools of susceptible and resistant recombinant inbred lines was used to specifically target the mechanisms for tolerance. Differential abundances were evidenced for proteins involved in defence (e.g., PR5, PR10, or Kunitz protease inhibitors) and signalling pathways (such as cyclophilin). <fi>R.?solanacearum</fi> inoculation modifies expression levels of those genes, either in both genotypes (AOS1, LOX4, and proteinase inhibitors) or specifically in the resistant line (PR proteins). Exogenous application of salicylic acid (SA) enhanced tolerance to the bacteria, whereas methyl jasmonate (MeJA) enhanced short‐term tolerance then promoted disease in the susceptible ecotype, suggesting that they may mediate defence responses. Conversely, proteomics‐identified genes were also shown to be SA or MeJA responsive. This is the first description of differential response to <fi>R.?solanacearum</fi> in <fi> <fc>M.?truncatula</fc> . </fi> Our results suggest that root basal defence is activated at 1?dpi, together with the JA pathway. Specific resistance is then evidenced at three dpi, with the up‐regulation of SA‐dependent PR proteins. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >模型豆类<fc> <fi> medicago truncatula </ fi> < / fc> <fi> ralstonia solanacearum </ fi> gmi1000通过根系殖民化引起细菌枯萎病。对早期感染的根和叶响应（1和3？天后感染后）的特征是研究植物抗性或易感性的分子机制。使用基于易感和抗性重组近交系池的蛋白质组学方法来特异性地靶向耐受性的机制。证明了差异丰度用于防御的蛋白质（例如，PR5，PR10或Kunitz蛋白酶抑制剂）和信号传导途径（例如环旋蛋白）。 <fi> r.?solanacearum </ fi>接种在基因型（AOS1，LOX4和蛋白酶抑制剂）中或特异性地修饰这些基因的表达水平，或者特别是在抗性管线（PR蛋白）中。外源性水杨酸（SA）增强对细菌的耐受性，而茉莉酸甲酯（Meja）增强短期耐受性，然后在易感生态型中促进疾病，这表明他们可能会调解辩护反应。相反，蛋白质组学鉴定的基因也显示为SA或MEJA响应。这是对<fi> r.?solanacearum </ fi>中的差分响应的第一个描述<fi> <fc> m.?truncatula </ fc>。 </ Fi>我们的结果表明，根基防御在1？DPI与JA路径一起激活。然后在三次DPI下证明特异性抗性，具有依赖于SA依赖性PR蛋白的上调。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15891/'>《Cellular microbiology》</a> <b style="margin: 0 2px;">|</b><span>2018年第4期</span><b style="margin: 0 2px;">|</b><span>共16页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yamchi Ahad&option=202" target="_blank" rel="nofollow">Yamchi Ahad;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ben Cécile&option=202" target="_blank" rel="nofollow">Ben Cécile;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rossignol Michel&option=202" target="_blank" rel="nofollow">Rossignol Michel;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zareie Sayed Reza&option=202" target="_blank" rel="nofollow">Zareie Sayed Reza;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mirlohi Aghafakhr&option=202" target="_blank" rel="nofollow">Mirlohi Aghafakhr;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sayed‐Tabatabaei Badraldin Ebrahim&option=202" target="_blank" rel="nofollow">Sayed‐Tabatabaei Badraldin Ebrahim;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pichereaux Carole&option=202" target="_blank" rel="nofollow">Pichereaux Carole;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sarrafi Ahmad&option=202" target="_blank" rel="nofollow">Sarrafi Ahmad;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rickauer Martina&option=202" target="_blank" rel="nofollow">Rickauer Martina;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gentzbittel Laurent&option=202" target="_blank" rel="nofollow">Gentzbittel Laurent;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Plant Breeding and BiotechnologyGorgan University of Agricultural Sciences and Natural ResourcesGorgan Iran;</p> <p>EcoLabUniversité de Toulouse CNRS INPT UPSToulouse France;</p> <p>Institut de Pharmacologie et de Biologie StructuraleUniversite de Toulouse IFR40 Plateforme Protéomique du Génopole Toulouse Midi‐PyrénéesCNRS UMR 5089 31077 Toulouse France;</p> <p>Department of Agricultural biotechnology College of AgricultureIsfahan University of Technology84156‐83111 Isfahan Iran;</p> <p>Department of Agricultural biotechnology College of AgricultureIsfahan University of Technology84156‐83111 Isfahan Iran;</p> <p>Department of Agricultural biotechnology College of AgricultureIsfahan University of Technology84156‐83111 Isfahan Iran;</p> <p>Institut de Pharmacologie et de Biologie StructuraleUniversite de Toulouse IFR40 Plateforme Protéomique du Génopole Toulouse Midi‐PyrénéesCNRS UMR 5089 31077 Toulouse France;</p> <p>EcoLabUniversité de Toulouse CNRS INPT UPSToulouse France;</p> <p>EcoLabUniversité de Toulouse CNRS INPT UPSToulouse France;</p> <p>EcoLabUniversité de Toulouse CNRS INPT UPSToulouse France;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/173.html" title="普通生物学">普通生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=barrel medic&option=203" rel="nofollow">barrel medic;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=hydroponic culture&option=203" rel="nofollow">hydroponic culture;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=proteomics&option=203" rel="nofollow">proteomics;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=RILs&option=203" rel="nofollow">RILs;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=root disease&option=203" rel="nofollow">root disease;</a> </p> <div class="translation"> 机译：桶军医;水培培养;蛋白质组学;rils;根病; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div 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style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于NFC的Wi-Fi自动连接服务的移动终端位置信息交互传输方法 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130410020267.html">System using NFC, Bluetooth, and/or Wi-Fi Technologies for Making a Quick Connection between a Smart Mobile Electronic Device and Audio Speakers, with an Optional Video Display System</a> <b>[P]</b> . <span> 外国专利： <!-- 美国专利： --> US2017180915A1 </span> <span> . 2017-06-22</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用NFC，蓝牙和/或Wi-Fi技术的系统，用于在智能移动电子设备和音频扬声器之间进行快速连接，并带有可选的视频显示系统 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> 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