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The bile salt sodium taurocholate induces Campylobacter jejuniCampylobacter jejuni outer membrane vesicle production and increases OMV‐associated proteolytic activity

机译：胆汁盐牛磺酸钠诱导弯曲杆菌jejuni Campylobacter Jejuni外膜囊泡产生并增加OMV相关的蛋白水解活性

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Abstract > <fi>Campylobacter jejuni</fi> , the leading cause of bacterial acute gastroenteritis worldwide, secretes an arsenal of virulence‐associated proteins within outer membrane vesicles (OMVs). <fi>C</fi> .? <fi>jejuni</fi> OMVs contain three serine proteases (HtrA, Cj0511, and Cj1365c) that cleave the intestinal epithelial cell (IEC) tight and adherens junction proteins occludin and E‐cadherin, promoting enhanced <fi>C</fi> .? <fi>jejuni</fi> adhesion to and invasion of IECs. <fi>C</fi> .? <fi>jejuni</fi> OMVs also induce IECs innate immune responses. The bile salt sodium taurocholate (ST) is sensed as a host signal to coordinate the activation of virulence‐associated genes in the enteric pathogen <fi>Vibrio cholerae.</fi> In this study, the effect of ST on <fi>C</fi> .? <fi>jejuni</fi> OMVs was investigated. Physiological concentrations of ST do not have an inhibitory effect on <fi>C</fi> .? <fi>jejuni</fi> growth until the early stationary phase. Coculture of <fi>C</fi> .? <fi>jejuni</fi> with 0.1% or 0.2% ( <fi>w</fi> / <fi>v</fi> ) ST stimulates OMV production, increasing both lipid and protein concentrations. <fi>C</fi> .? <fi>jejuni</fi> ST‐OMVs possess increased proteolytic activity and exhibit a different protein profile compared to OMVs isolated in the absence of ST. ST‐OMVs exhibit enhanced cytotoxicity and immunogenicity to T84 IECs and enhanced killing of <fi>Galleria mellonella</fi> larvae. ST increases the level of mRNA transcripts of the OMVs‐associated serine protease genes and the <fi>cdtABC</fi> operon t </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> > <fi> campylobacter jejuni </ fi，细菌急性胃肠炎全球的主要原因，分泌外膜囊泡（OMV）内的毒力相关蛋白的阿森纳。 <fi> c </ fi>。？ <fi> jejuni </ fi> omvs含有三种丝氨酸蛋白酶（HTRA，CJ0511和CJ1365C），可切割肠上皮细胞（IEC）紧密和粘附结蛋白闭塞蛋白和E-Cadherin，促进增强型<Fi> C </ Fi >。？ <fi> jejuni </ fi> IECs的粘附和入侵。 <fi> c </ fi>。？ <fi> jejuni </ fi> omvs还诱导IECs先天免疫应答。将胆汁盐钠钠盐钠（ST）称为宿主信号，以协调肠道病原体中的毒力相关基因的激活<fi> vibrio cholerae。</ fi>在本研究中，st对<fi> c的影响</ fi>。？ <fi> jejuni </ fi> OMV被调查。 ST的生理浓度对<fi> c </ fi>没有抑制作用。 <fi> jejuni </ fi>增长直到早期的固定阶段。 <fi> c </ fi>的共生。？ <fi> jejuni </ fi> 0.1％或0.2％（<fi> w </ fi> / <fi> v </ fi>）st刺激omv产生，增加脂质和蛋白质浓度。 <fi> c </ fi>。？ <fi> jejuni </ fi> st-omvs具有增加的蛋白水解活性，与在没有st的情况下分离的omvs相比表现出不同的蛋白质剖面。 ST-OMVs表现出增强的细胞毒性和免疫原性至T84 IECs，增强了<FI> Galleria Mellonella </ Fi>幼虫的杀伤。 ST增加了OMVS相关丝氨酸蛋白酶基因的mRNA转录物的水平和<FI> CDTABC </ FI>操纵杆 </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15891/'>《Cellular microbiology》</a> <b style="margin: 0 2px;">|</b><span>2018年第3期</span><b style="margin: 0 2px;">|</b><span>共14页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Elmi Abdi&option=202" target="_blank" rel="nofollow">Elmi Abdi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dorey Amber&option=202" target="_blank" rel="nofollow">Dorey Amber;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Watson Eleanor&option=202" target="_blank" rel="nofollow">Watson Eleanor;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jagatia Heena&option=202" target="_blank" rel="nofollow">Jagatia Heena;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Inglis Neil F.&option=202" target="_blank" rel="nofollow">Inglis Neil F.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gundogdu Ozan&option=202" target="_blank" rel="nofollow">Gundogdu Ozan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bajaj‐Elliott Mona&option=202" target="_blank" rel="nofollow">Bajaj‐Elliott Mona;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wren Brendan W.&option=202" target="_blank" rel="nofollow">Wren Brendan W.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Smith David G.E.&option=202" target="_blank" rel="nofollow">Smith David G.E.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dorrell Nick&option=202" target="_blank" rel="nofollow">Dorrell Nick;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Faculty of Infectious &</p> <p>Tropical DiseasesLondon School of Hygiene &</p> <p>Tropical MedicineLondon UK;</p> <p>Faculty of Infectious &</p> <p>Tropical DiseasesLondon School of Hygiene &</p> <p>Tropical MedicineLondon UK;</p> <p>Moredun Research InstitutePenicuik UK;</p> <p>Faculty of Infectious &</p> <p>Tropical DiseasesLondon School of Hygiene &</p> <p>Tropical MedicineLondon UK;</p> <p>Moredun Research InstitutePenicuik UK;</p> <p>Faculty of Infectious &</p> <p>Tropical DiseasesLondon School of Hygiene &</p> <p>Tropical MedicineLondon UK;</p> <p>Infection Immunity Inflammation and Physiological MedicineUCL Institute of Child HealthLondon UK;</p> <p>Faculty of Infectious &</p> <p>Tropical DiseasesLondon School of Hygiene &</p> <p>Tropical MedicineLondon UK;</p> <p>School of Engineering &</p> <p>Physical SciencesHeriot‐Watt UniversityEdinburgh UK;</p> <p>Faculty of Infectious &</p> <p>Tropical DiseasesLondon School of Hygiene &</p> <p>Tropical MedicineLondon UK;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/173.html" title="普通生物学">普通生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=bile salts&option=203" rel="nofollow">bile salts;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Campylobacter&option=203" rel="nofollow">Campylobacter;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=outer membrane vesicles&option=203" rel="nofollow">outer membrane vesicles;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=proteolytic activity&option=203" rel="nofollow">proteolytic activity;</a> </p> <div class="translation"> 机译：胆汁;弯曲杆菌;外膜囊泡;蛋白水解活性; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >专利</li> </ul> </div> <div 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<div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130446955543.html">Monoclonal antibodies against Campylobacter jejuni and Campylobacter coli outer membrane antigens</a> <b>[P]</b> . <span> 外国专利：  US6395879B1 </span> <span> . 2002-05-28</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：空肠弯曲菌和大肠弯曲菌外膜抗原的单克隆抗体 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130447044931.html">Monoclonal antibodies against campylobacter jejuni and campylobacter coli outer membrane antigens</a> <b>[P]</b> . <span> 外国专利：  US2002106383A1 </span> <span> . 2002-08-08</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：空肠弯曲菌和大肠杆菌弯曲菌外膜抗原的单克隆抗体 </span> </p> </li> </ul> </div> </div> </div> <div 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