Recombinant Bacillus subtilis 26DCryChS line with gene Btcry1Ia encoding Cry1Ia toxin from Bacillus thuringiensis promotes integrated wheat defense against pathogen Stagonospora nodorum Berk. and greenbug Schizaphis graminum Rond

摘要

The novel recombinant Bacillus subtilis 26DCryChS line that contains the Bacillus thuringiensis B-5351 strain gene Btcry1Ia encoding Cry1Ia toxin is described. B. subtilis 26DCryChS line kept endophytic properties and ability to synthesize lipopeptide surfactin of B. subtilis 26D strain and acquired the capability to produce Cry1Ia protein from B. thuringiensis strain B-5351. Insecticidal activity of lipopeptide-rich fraction of cultural filtrate of B. subtilis 26D strain and B. subtilis 26DCryChS line, which was identical to commercial surfactin, against greenbug Schizaphis graminum Rond was shown for the first time. Furthermore, B. subtilis 26DCryChS as much as original B. subtilis 26D strain inhibited growth of fungal wheat pathogen Stagonospora nodorum Berk. Bas1. Insertion of the gene Btcry1Ia in genome of B. subtilis 26D strain contributed to increase of aphicidal activity of B. subtilis 26DCryChS line against S. graminum as compared with both B. thuringiensis B-5351 and B. subtilis 26D strains.