Interoperable RNA-Seq analysis in the cloud

摘要

RNA-Sequencing (RNA-Seq) is currently the leading technology for genome-wide transcript quantification. Mapping the raw reads to transcript and gene level counts can be achieved by different aligners. Here we report an in-depth comparison of transcript quantification methods. Our goal is the specific use of cost-efficient RNA-Seq analysis for deployment in a cloud infrastructure composed of interacting microservices. The individual modules cover file transfer into the cloud and APIs to handle the cloud alignment jobs. We next demonstrate how newly generated RNA-Seq data can be placed in the context of thousands of previously published datasets in near real time. With in-depth benchmarks, we identify suitable gene count quantification methods to facilitate cost-effective, accurate, and cloud-based RNA-Seq analysis service. Pseudo-alignment algorithms such as kallisto and Salmon combine high read quality estimation with cost efficient runtime performance. HISAT2 is the fastest of the classical aligners with good alignment quality.