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首页> 外文期刊>Biotechnology and Bioengineering >Enhanced isobutanol production from acetate by combinatorial overexpression of acetyl‐CoA synthetase and anaplerotic enzymes in engineered Escherichia coliEscherichia coli
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Enhanced isobutanol production from acetate by combinatorial overexpression of acetyl‐CoA synthetase and anaplerotic enzymes in engineered Escherichia coliEscherichia coli

机译：通过Compinatoral过表达通过乙酰-CoA合成酶和工程化酶的组合过表达增强异丁醇生产大肠杆菌大肠杆菌

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Abstract <section xml:id="bit26710-sec-0001" numbered="no"> > Acetic acid is an abundant material that can be used as a carbon source by microorganisms. Despite its abundance, its toxicity and low energy content make it hard to utilize as a sole carbon source for biochemical production. To increase acetate utilization and isobutanol production with engineered Escherichia coli , the feasibility of utilizing acetate and metabolic engineering was investigated. The expression of acs, pckA , and maeB increased isobutanol production by up to 26%, and the addition of TCA cycle intermediates indicated that the intermediates can enhance isobutanol production. For isobutanol production from acetate, acetate uptake rates and the NADPH pool were not limiting factors compared to glucose as a carbon source. This work represents the first approach to produce isobutanol from acetate with pyruvate flux optimization to extend the applicability of acetate. This technique suggests a strategy for biochemical production utilizing acetate as the sole carbon source. </section> </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> <section XML：ID =“Bit26710-SEC-0001”编号=“否”> >乙酸是一种丰富的材料，可用微生物用作碳源。尽管其丰富，其毒性和低能量含量使其难以作为生化生产的唯一碳源。为了提高醋酸酯利用率和异丁醇生产，通过工程化大肠杆菌，采用醋酸盐和代谢工程的可行性进行了研究。 Acs，Pcka </ i>和 Maeb </ i>的表达增加了脱丁醇产量，高达26％，加入TCA循环中间体表明中间体可以增强异丁醇生产。对于醋酸盐的异丁醇生产，与葡萄糖作为碳源相比，醋酸乙酸碱基和NADPH池没有限制因子。该作品代表了生产丙酮酸助焊剂优化的醋酸异丁醇的第一种方法，以延长乙酸酯的适用性。该技术表明，利用醋酸盐作为唯一碳源的生物化学生产策略。 </ p> </ section> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15017/'>《Biotechnology and Bioengineering》</a> <b style="margin: 0 2px;">|</b><span>2018年第8期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Song Hun‐Suk&option=202" target="_blank" rel="nofollow">Song Hun‐Suk;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Seo Hyung‐Min&option=202" target="_blank" rel="nofollow">Seo Hyung‐Min;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jeon Jong‐Min&option=202" target="_blank" rel="nofollow">Jeon Jong‐Min;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Moon Yu‐Mi&option=202" target="_blank" rel="nofollow">Moon Yu‐Mi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hong Ju Won&option=202" target="_blank" rel="nofollow">Hong Ju Won;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hong Yoon Gi&option=202" target="_blank" rel="nofollow">Hong Yoon Gi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bhatia Shashi Kant&option=202" target="_blank" rel="nofollow">Bhatia Shashi Kant;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ahn Jungoh&option=202" target="_blank" rel="nofollow">Ahn Jungoh;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lee Hongweon&option=202" target="_blank" rel="nofollow">Lee Hongweon;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kim Wooseong&option=202" target="_blank" rel="nofollow">Kim Wooseong;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Park Yong‐Cheol&option=202" target="_blank" rel="nofollow">Park Yong‐Cheol;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Choi Kwon‐Young&option=202" target="_blank" rel="nofollow">Choi Kwon‐Young;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kim Yun‐Gon&option=202" target="_blank" rel="nofollow">Kim Yun‐Gon;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Yung‐Hun&option=202" target="_blank" rel="nofollow">Yang Yung‐Hun;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Biological EngineeringKonkuk UniversitySeoul Korea;</p> <p>Department of Biological EngineeringKonkuk UniversitySeoul Korea;</p> <p>Department of Biological EngineeringKonkuk UniversitySeoul Korea;</p> <p>Department of Biological EngineeringKonkuk UniversitySeoul Korea;</p> <p>Department of Biological EngineeringKonkuk UniversitySeoul Korea;</p> <p>Department of Biological EngineeringKonkuk UniversitySeoul Korea;</p> <p>Department of Biological EngineeringKonkuk UniversitySeoul Korea;</p> <p>Biotechnology Process Engineering CenterKorea Research Institute Bioscience Biotechnology (KRIBB)Daejeon Korea;</p> <p>Biotechnology Process Engineering CenterKorea Research Institute Bioscience Biotechnology (KRIBB)Daejeon Korea;</p> <p>Division of Infectious DiseasesAlpert Medical School of Brown UniversityProvidence Rhode Island;</p> <p>Department of Bio and Fermentation Convergence TechnologyKookmin UniversitySeoul Republic of Korea;</p> <p>Department of Environmental EngineeringAjou UniversitySuwon Gyeonggi‐do Republic of Korea;</p> <p>Department of Chemical EngineeringSoongsil UniversitySeoul Republic of Korea;</p> <p>Department of Biological EngineeringKonkuk UniversitySeoul Korea;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=acetate&option=203" rel="nofollow">acetate;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=anaplerotic enzymes&option=203" rel="nofollow">anaplerotic enzymes;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Escherichia coli&option=203" rel="nofollow">Escherichia coli;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=isobutanol&option=203" rel="nofollow">isobutanol;</a> </p> <div class="translation"> 机译：醋酸盐;血栓酶;大肠杆菌;异丁醇; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704021882289.html">Enhanced isobutanol production from acetate by combinatorial overexpression of acetyl‐CoA synthetase and anaplerotic enzymes in engineered <i xmlns="http://www.wiley.com/namespaces/wiley">Escherichia coliEscherichia coli</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Song Hun‐Suk&option=202" target="_blank" rel="nofollow" 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