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首页> 外文期刊>Biotechnology and Bioengineering >Ultrahigh‐cell‐density heterotrophic cultivation of the unicellular green microalga Scenedesmus acuminatusScenedesmus acuminatus and application of the cells to photoautotrophic culture enhance biomass and lipid production
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Ultrahigh‐cell‐density heterotrophic cultivation of the unicellular green microalga Scenedesmus acuminatusScenedesmus acuminatus and application of the cells to photoautotrophic culture enhance biomass and lipid production

机译：超高细胞密度单细胞绿色微藻的异养培养 scenedesmus acuminatus scenedesmus acuminatus 细胞在光射营养培养物中的应用增强生物质和脂质生产

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Abstract > Although production of biodiesels from microalgae is proved to be technically feasible, a commercially viable system has yet to emerge. High‐cell‐density fermentation of microalgae can be coupled with photoautotrophic cultivation to produce oils. In this study, by optimizing culturing conditions and employing a sophisticated substrate feed control strategy, ultrahigh‐cell‐density of 286 and 283.5?g/L was achieved for the unicellular alga Scenedesmus acuminatus grown in 7.5‐L bench‐scale and 1,000‐L pilot‐scale fermenters, respectively. The outdoor scale‐up experiments indicated that heterotrophically grown S . acuminatus cells are more productive in terms of both biomass and lipid accumulation when they are inoculated in photobioreactors for lipid production as compared to the cells originally grown under photoautotrophic conditions. Technoeconomic analysis based on the pilot‐scale data indicated that the cost of heterotrophic cultivation of microalgae for biomass production is comparable with that of the open‐pond system and much lower than that of tubular PBR, if the biomass yield was higher than 200?g/L. This study demonstrated the economic viability of heterotrophic cultivation on large‐scale microalgal inocula production, but ultrahigh‐productivity fermentation is a prerequisite. Moreover, the advantages of the combined heterotrophic and photoautotrophic cultivation of microalgae for biofuels production were also verified in the pilot‐scale. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 虽然从微藻生产的生物柴油的生产被证明是技术上可行的，但商业上可行的系统尚未出现。微藻的高电池密度发酵可以与光光营养培养相结合以生产油。在本研究中，通过优化培养条件并采用复杂的基质进料控制策略，为单细胞藻类实现了286和283.5μm的超高电池密度 scenedesmus acuminatus </ i> 分别生长在7.5-l台阶和1,000-L先导式发酵罐中。室外扩展实验表明，异冰地生长 s </ i> 。 acuminatus </ i> 当与最初在光摄制条件下生长的细胞相比，在生物量和脂质积累时，细胞在生物质和脂质积累方面更高效。基于导频数据的技术经济分析表明，如果生物质产量高于200μmΩ·g / l。本研究表明，异养培养对大规模微藻造影的经济可行性，但超高产量发酵是一种先决条件。此外，在试验规模中还验证了用于生物燃料生物燃料的微观营养和光学营养的优点。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15017/'>《Biotechnology and Bioengineering》</a> <b style="margin: 0 2px;">|</b><span>2020年第1期</span><b style="margin: 0 2px;">|</b><span>共13页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=heterotrophy&option=203" rel="nofollow">heterotrophy;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=high‐cell‐density fermentation&option=203" rel="nofollow">high‐cell‐density fermentation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=lipid&option=203" rel="nofollow">lipid;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=microalgae&option=203" rel="nofollow">microalgae;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Scenedesmus acuminatus&option=203" rel="nofollow">Scenedesmus acuminatus;</a> </p> <div class="translation"> 机译：异营养;高细胞密度发酵;脂质;微藻;Scenedesmus Acuminatus; </div> </li> <li> <span class="lefttit">入库时间</span> <span>2022-08-19 23:00:54</span> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704021881519.html">Ultrahigh‐cell‐density heterotrophic cultivation of the unicellular green microalga <i >Scenedesmus acuminatusScenedesmus acuminatus and application of the cells to photoautotrophic culture enhance biomass and lipid production</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" 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