Cofactor‐embedded nanoporous activated carbon matrices for the immobilization of intracellular enzymes and degradation of endocrine disruptor

Paranji Saranya; Ganesan Sekaran

摘要

Abstract > The mixed intracellular enzyme (MICE) from Citrobacter freundii , capable of degrading o‐phenylene diamine (OPD), was extracted and characterized. Cofactors such as zinc and copper ions enhanced the MICE activity. The functionalized nanoporous‐activated carbon (FNAC) matrix, zinc‐impregnated FNAC matrix (Zn 2+ ‐FNAC), copper‐impregnated FNAC matrix (Cu 2+ ‐FNAC), and zinc‐ and copper‐impregnated FNAC matrix (Zn 2+ ‐Cu 2+ ‐FNAC) were prepared and characterized to immobilize MICE. The parameters such as time (0–240 Min), pH (1–10), temperature (20–50?oC), amount of MICE (1–5 mg), particle size of carbon (100–600 μm), and mass of carbon (0.5–2.5 g) were optimized for immobilization of MICE on different FNAC matrices. The carrier matrices in the free and MICE immobilized form were characterized using SEM, FT‐IR, XPS, XRD, thermogravimetric analysis (TGA), and DSC analyses. The kinetic and adsorption models for the immobilization of MICE on FNAC matrices were studied. The parameters such as time, pH, temperature, concentration of OPD, and agitation speed were optimized for the degradation of OPD using FNAC‐MICE and MICE‐immobilized metal‐impregnated FNAC matrices. The maximum amount of pyruvic acid formed was found to be 133 μg/mg of OPD using Zn 2+ ‐Cu 2+ ‐FNAC‐MICE matrix. The kinetic models were studied for the formation of pyruvic acid on OPD degradation and confirmed using FT‐IR spectroscopy. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”> <title type =“main”>抽象</ title> >来自<施用和表征能够降解O-苯基二胺（OPD）的柑橘杆菌Freundii </ i>。锌和铜离子等辅助耦接力增强了小鼠活性。官能化纳米孔活性炭（FNAC）基质，锌浸渍的FNAc基质（Zn <Sup> 2 + </ sup> -FNAc），铜 - 浸渍的FNAc基质（Cu 2 + </ sup> -fnac）制备锌和铜浸渍的FNAC基质（锌浸渍的FNAC基质（Zn 2 + </ sup> -cu 2 + </ sup> -fnac）并表征以固定小鼠。时间（0-240分钟），pH（1-10），温度（20-50Ω·oc），小鼠（1-5mg），碳（100-600μm）的粒度（100-600μm）等参数，优化碳质量（0.5-2.5g）以固定在不同的FNAc基质上的小鼠。使用SEM，FT-IR，XPS，XRD，热重分析（TGA）和DSC分析，表征自由和小鼠固定的形式中的载体基质。研究了对FNAc基质固定小鼠的动力学和吸附模型。使用FNAC-小鼠和小鼠固定的金属浸渍的FNAC基质，优化了OPD的时间，pH，温度，OPD的浓度和搅拌速度的参数。使用Zn 2 + / sop> -cu 2 + / sop> -fnac-mice基质，发现形成形成的丙酮酸的最大量为133μg/ mg OPD。研究了动力学模型，用于在OPD降解上形成丙酮酸并使用FT-IR光谱证实。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15016/'>《Biotechnology and Applied Biochemistry》</a> <b style="margin: 0 2px;">|</b><span>2017年第3期</span><b style="margin: 0 2px;">|</b><span>共21页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Paranji Saranya&option=202" target="_blank" rel="nofollow">Paranji Saranya;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ganesan Sekaran&option=202" target="_blank" rel="nofollow">Ganesan Sekaran;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Environmental Technology DivisionCouncil of Scientific and Industrial Research (CSIR) Central Leather Research Institute (CLRI)Adyar Chennai India;</p> <p>Environmental Technology DivisionCouncil of Scientific and Industrial Research (CSIR) Central Leather Research Institute (CLRI)Adyar Chennai India;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/177.html" title="生物化学">生物化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=o‐phenylene diamine&option=203" rel="nofollow">o‐phenylene diamine;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=immobilized intracellular enzymes&option=203" rel="nofollow">immobilized intracellular enzymes;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=functionalized nanoporous‐activated carbon&option=203" rel="nofollow">functionalized nanoporous‐activated carbon;</a> </p> <div class="translation"> 机译：O-苯基二胺;固定化细胞内酶;官能化纳米多孔活性炭; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 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Part 4. New Methods, Enzymes, and Approaches</a> <b>[R] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wingard, L. B.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wingard, L. B.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Castner, J. F.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Castner, J. F.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Miyawaki, O.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Miyawaki, O.,</a> <span>1984</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：旋转环盘电极：用于固定化酶载体基质研究质量传递阻力。第4部分。新方法，酶和方法</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201295360211.html">固定化微生物降解环境内分泌干扰物DBP</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王琳&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 王琳</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=罗启芳&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,罗启芳</a> <span> <a href="/journal-cn-56771/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国公共卫生 </a> </span> <span> . 2003</span><span>,第11期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-traumatic-surgery_thesis/0201233500014.html">可降解镁锌合金肋骨固定器应用于肋骨骨折内固定的动物实验研究</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=秦雄&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 秦雄</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=闵捷&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,闵捷</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈熹&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,陈熹</a> <span> <a href="/journal-cn-6074/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 创伤外科杂志 </a> </span> <span> . 2016</span><span>,第002期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" 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