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首页> 外文期刊>Biotechnology and Applied Biochemistry >Structural basis of noncanonical polyphenol oxidase activity in DLL‐II: A lectin from Dolichos lablabDolichos lablab
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Structural basis of noncanonical polyphenol oxidase activity in DLL‐II: A lectin from Dolichos lablabDolichos lablab

机译：DLL-II中非碳多酚氧化酶活性的结构基础：来自 dolichos lablab dolichos lablab的凝集素

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Abstract > Lectins known to possess an additional enzymatic function are called leczymes. Previous studies reported a unique polyphenol oxidase (PPO) activity in DLL‐II—a leczyme from Dolichos lablab . DLL‐II shares a high sequence and structural homology with DBL—another leczyme from Dolichos biflorus . Incidentally, DBL possesses lipoxygenase activity, but not the PPO activity. Legume lectins usually possess two metal‐binding sites A and B. Although these sites are conserved in both DBL and DLL‐II, site A in DLL‐II is occupied by Mn 2+ and site B by Ca 2+ . In contrast, DLL‐II binds Cu 2+ and Ca 2+ at sites A and B, respectively. Here, investigating the structural basis of PPO activity in DLL‐II, we find that the PPO activity is only dependent on Cu 2+ , but not Ca 2+ ; and the lectin activity requires only Ca 2+ . Further, our analysis suggests that an alternative mechanism of PPO reaction may be operative in DLL‐II, which involves a mononuclear Cu 2+ metal center; this is in contrast to the bi‐nuclear Cu 2+ metal center commonly observed in all PPOs. Importantly, structural and computational approaches employed here, we hypothesize possible PPO binding sites and the corresponding migration channels for accessing the active site. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >章参具有额外的酶促功能称为leczymes。以前的研究报告了来自 Dolichos Lablab的DLL-II-A leczyme中的独特的多酚氧化酶（PPO）活性。 DLL-II与来自 Dolichos Biflorus的DBL-Adjer leczyme共享高序列和结构同源性。顺便提及，DBL具有脂氧合酶活性，但不是PPO活性。豆类凝集素通常具有两个金属结合位点A和B.虽然这些位点在DBL和DLL-II中保守，但DLL-II中的部位A由Mn <Sup> 2 + </ sup>和网站B占据 2 + </ sup>。相反，DLL-II分别在位点A和B的Cu 2 + </ sop>和Ca 2 + / sup>。这里，研究DLL-II中PPO活性的结构基础，我们发现PPO活性仅取决于Cu 2 + </ sup>，但不是ca 2 + </ sup>;并且凝集素活动只需要CA <SUP> 2 + </ sup>。此外，我们的分析表明，PPO反应的替代机制可以在DLL-II中携带，其涉及单核Cu 2 + / sup>金属中心;这与在所有PPO中通常观察到的双核Cu 2 + / sup>金属中心相反。重要的是，这里采用的结构和计算方法，我们假设可能的PPO绑定站点和用于访问活动站点的相应迁移信道。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15016/'>《Biotechnology and Applied Biochemistry》</a> <b style="margin: 0 2px;">|</b><span>2018年第5期</span><b style="margin: 0 2px;">|</b><span>共17页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=L. Vishweshwaraiah Yashavanth&option=202" target="_blank" rel="nofollow">L. Vishweshwaraiah Yashavanth;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Acharya Abhishek&option=202" target="_blank" rel="nofollow">Acharya Abhishek;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Prakash Balaji&option=202" target="_blank" rel="nofollow">Prakash Balaji;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Molecular NutritionCSIR‐Central Food Technological Research InstituteMysore India;</p> <p>Department of Molecular NutritionCSIR‐Central Food Technological Research InstituteMysore India;</p> <p>Department of Molecular NutritionCSIR‐Central Food Technological Research InstituteMysore India;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/177.html" title="生物化学">生物化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=leczyme&option=203" rel="nofollow">leczyme;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=polyphenol oxidase&option=203" rel="nofollow">polyphenol oxidase;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=bi‐functional lectins&option=203" rel="nofollow">bi‐functional lectins;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=tunnel computation&option=203" rel="nofollow">tunnel computation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=docking&option=203" rel="nofollow">docking;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=substrate binding&option=203" rel="nofollow">substrate binding;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=metallozymes&option=203" rel="nofollow">metallozymes;</a> </p> <div class="translation"> 机译：leczyme;聚酚氧化酶;双功能凝集素;隧道计算;对接;衬底结合;金属; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704021881045.html">Structural basis of noncanonical polyphenol oxidase activity in DLL‐II: A lectin from <i xmlns="http://www.wiley.com/namespaces/wiley">Dolichos lablabDolichos lablab</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=L. 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