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Novel SNPs of the mtDNA ND5 gene and their associations with several growth traits in the Nanyang cattle breed.

机译:MTDNA ND5基因的新型SNP及其与南洋牛品种的几种生长特征的关联。

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摘要

Polymorphism of the mtDNA ND5 gene was detected by PCR-SSCP and DNA sequencing methods in 714 individuals from six Chinese cattle breeds. The results showed that at the P2 locus, two kinds of haplotypes, named A and B, with three SNPs (T12900C, A12923T, C12924T) were detected. The frequencies of haplotype A in six breeds were 0.041-0.167. Polymorphism of the ND5 gene was shown to be associated with growth in the Nanyang breed. Individuals with haplotype B had greater hucklebone width than those with haplotype A when 6 months old (P < 0.01), as well as better body height, body length, body weight, and average daily gain at 6 months (P < 0.05). Therefore, haplotype B of the ND5 gene was likely to have a positive effect on growth traits at 6 months in the Nanyang breed.
机译:通过PCR-SSCP和DNA测序方法在714个中来自六种中国牧场品种的单独的PCR-SSCP和DNA测序方法检测MTDNA ND5基因的多态性。 结果表明,在P2基因座,检测到两种单倍型,命名A和B,具有三个SNP(T12900C,A12923T,C12924T)。 单倍型A六种品种的频率为0.041-0.167。 显示ND5基因的多态性与南洋品种的生长有关。 具有单倍型B的个体具有更大的Hucklebone宽度,而不是单倍型A的6个月(P <0.01),以及更好的身体高度,体长,体重和6个月平均每日增益(P <0.05)。 因此,ND5基因的单倍型B可能对南洋繁殖的6个月产生积极影响生长性状。

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