INCORPORATION OF LEAF RUST RESISTANCE GENES INTO WINTER WHEAT GENOTYPES USING MARKER-ASSISTED SELECTION

摘要

The breeding and cultivation of resistant wheat varieties is an effective way ofcontrolling leaf rust (Puccinia triticina Eriks.)- The use of molecular markers facilitates theincorporation of the major leaf rust resistance genes (Lr genes) responsible for resistanceinto new varieties and the pyramiding of these genes. Marker-assisted selection was usedto incorporate the Lr genes currently effective in Hungary (Lr9, Lr24, Lr25, Lr29) intowinter wheat varieties. The Lr genes were identified using STS, SCAR and RAPD markersclosely linked to them. Investigations were made on how these markers could be utilised inplant breeding, and near-isogenic lines resembling the recurrent variety but eachcontaining a different Lr gene were developed to form the initial stock for the pyramidingof resistance genes. The results indicate that the marker-assisted selection techniqueelaborated for resistance genes Lr24, Lr25 and Lr29 can be applied simply and effectivelyin wheat breeding, while the detection of the Lr9 marker is uncertain.