INDUCTION OF WHEAT/BARLEY TRANSLOCATIONS BY IRRADIATION AND THEIR DETECTION USING FLUORESCENCE IN SITU HYBRIDIZATION

摘要

The aim of the present study was to test the efficiency of gamma irradiation in inducing translocations between wheat and barley genomes using addition lines. The Martonvasari 9 krl-Igri disomic addition set, previously produced in Martonvasar, was irradiated with gamma rays. The pattern of irradiation-induced intergenomic chromosome rearrangements was analysed in the mutagenized (M-0) generation by genomic in situ hybridization (GISH). Centric fusions and a wide variety of reciprocal, terminal and interstitial translocations were frequently induced. The intergeneric translocations produced here are expected to be stabilized in later backcross progenies as a set of introgression lines carrying few but distinct rearrangements.