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Homeostatically regulated synchronized oscillations induced by short-term tetrodotoxin treatment in cultured neuronal network

机译:培养神经元网络中短期河豚毒素治疗引起的稳态调节同步振荡

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摘要

Homeostatic plasticity plays a critical role in the stability of neuronal activities. Here, with high-density hippocampal networks cultured on multi-electrode arrays (MEAs), the transformation of spontaneous neuronal firing patterns induced by 1 mu M tetrodotoxin was clarified. Once tetrodotoxin was washed out after a 4-h treatment, spontaneous activities rose significantly with spike rate increasing approximately three times, and synchronized burst oscillations appeared throughout the network, with the cross-correlation coefficient between the active sites rising from 0.06 +/- 0.03 to 0.27 +/- 0.05. The long-term recording showed that the oscillations lasted for more than 4 h before the network recovered. These results suggest that short-term treatment by tetrodotoxin may induce the homeostatically enhanced neuronal excitability, and that the spontaneous synchronized oscillations should be an indicator of homeostatic plasticity in Cultured neuronal network. Furthermore, the non-invasive and long-term recording with MEAs as a novel sensing system is identified to be appropriate for pharmacological investigations of neuronal plasticity at the network level.
机译:稳态可塑性在神经元活动的稳定性中起关键作用。在这里,通过在多电极阵列(MEA)上培养的高密度海马网络,阐明了1μM河豚毒素诱导的自发神经元放电模式的转变。在经过4小时的处理后,河豚毒素被洗掉后,自发活动显着上升,峰值频率增加了大约三倍,并且整个网络中出现了同步爆发振荡,活动位点之间的互相关系数从0.06 +/- 0.03上升。至0.27 +/- 0.05。长期记录表明,在网络恢复之前,振荡持续了超过4小时。这些结果表明,河豚毒素的短期治疗可能诱导稳态增加的神经元兴奋性,并且自发同步振荡应该是培养的神经元网络中稳态可塑性的指标。此外,使用MEA作为一种新型传感系统的无创且长期记录被确定为适合在网络级别进行神经元可塑性的药理研究。

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