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Aromatic Compounds Production by Fungal Solid State Fermentation in Pandanus tectorius Fruits

机译:露兜树果实真菌固态发酵生产芳香族化合物

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Production of microbial aromatic compounds by solid-state fermentation is increasing lately, due to the ability of microbial to use agro-industrial wastes as their substrates. The aim of this research was to know potential of aromatic compounds production by solid-state fermentation of Trichoderma viridae in Pandanus tectorius fruits. To prepare the substrates, basal mesocarp part of fruits were cut, juiced, pulps were dried and grinded until reached fine particle size. Fermentations were carried outin 250 mL flasks with 10" fungal spores/mL, pH 6.0, 76,6 % (w/v) moistures at 30°C for 10 days. Aromatic compounds resulted from SSF were then analyzed using GC/MS. Results showed that Pandanus tectorius fruits contained total sugars 13,015%, proteins3%, lipids 1,39% and moisture content were 76,6%. This low sugars content in substrates seemed to be the main factor of Trichoderma viridae slow growth. There were 17 peaks of aromatic compounds detected in GC/MS, consisted of alkenes hydrocarbons (tetradecane, tertracosane, tetracosahexaene, pentadecane, hexacosane, heptadecane, and octadecane), alcohol (phenol), amide (9-octacenamide) and monoterpene aldehid (9-octadecenal). Therefore the fermentation conditions need to be further optimized to make better growth for fungi and higher aromatic compounds production.
机译:由于微生物利用农业工业废料作为其底物的能力,通过固态发酵的微生物芳香化合物的生产近来正在增加。这项研究的目的是了解在露兜树果实中固态木霉菌发酵产生芳香化合物的潜力。为了制备基质,将果实的中果皮部分切开,榨汁,将果肉干燥并研磨直至达到细粒度。在250 mL烧瓶中进行发酵,发酵条件为:250 mL烧瓶中的10“真菌孢子/ mL,pH 6.0,76.6%(w / v)水分,在30°C下发酵10天。然后使用GC / MS分析由SSF产生的芳香族化合物。结果表明,露兜树果实中的总糖含量为13,015%,蛋白质含量为3%,脂质含量为1,39%,水分含量为76.6%,底物中糖含量低似乎是木霉菌生长缓慢的主要因素,共有17个峰。在GC / MS中检测到的芳族化合物中,包括烯烃碳氢化合物(十四碳烷,叔二十烷,十四碳六烯,十五碳烷,十六烷,庚烷和十八烷),醇(苯酚),酰胺(9-十八烯酰胺)和单萜烯醛(9-十八烯醛)。因此,需要进一步优化发酵条件,以使真菌更好地生长并产生更高的芳香族化合物。

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