Sulfatide isoform pattern in cerebrospinal fluid discriminates progressive MS MS from relapsing‐remitting MS MS

摘要

Abstract Multiple sclerosis (MS ) is an inflammatory demyelinating disease of the central nervous system (CNS ). Several biomarkers including proteins and lipids have been reported inMS cerebrospinal fluid (CSF ), reflecting different aspects of the pathophysiology particularly of relapsingremittingMS (RRMS ). Sulfatide, abundant in the myelin sheath and a proposed target for autoimmune attack inMS , has been reported altered inMS CSF . Here, we investigated the potential ofCSF sulfatide and its isoforms as biomarkers inMS . A highly sensitive and quantitative mass spectrometry method was employed to determine levels of sulfatide isoforms inCSF fromRRMS and progressiveMS (PMS ) patients, and healthy donors (HD ). We demonstrate that levels of totalCSF sulfatide and C24:1, C26:1, and C26:1OH isoforms were significantly increased inPMS compared withRRMS patients andHD , while C23:0OH was significantly decreased inCSF fromPMS patients compared to the other two groups. Multivariate discriminant analysis showed thatCSF sulfatide isoform pattern inPMS patients was distinct and nonoverlapping with that ofRRMS patients andHD . Sulfatide levels did not correlate with tested biomarkers or clinical parameters. The results suggest thatCSF sulfatide isoform levels may be used to discriminate the phenotype of?MS and might play a role in the progression of the disease.