Online extraction of antihypertensive drugs and their metabolites from untreated human serum samples using restricted access carbon nanotubes in a column switching liquid chromatography system

摘要

Highlights?The RACNTs were able to extract drugs with different physicochemical properties.?The RACNTs excluded approximately 100% of proteins from human serum.?The RACNTs column presented a long lifetime.?The developed method was simple, reproducible and quick.?There were no carry-over and matrix effects in the analyses by UHPLC–MS/MS.AbstractA novel analytical method was developed to determine 5 antihypertensive drugs of different pharmacological classes (angiotensin-converting enzyme inhibitors, calcium channel blockers, α-2 adrenergic receptor agonists, angiotensin II receptor blockers, and aldosterone receptor antagonists) and some of their metabolites in human serum. The untreated samples were directly analyzed in a column switching system using an extraction column packed with restricte