Reaction engineering studies on biodegradation of phenol by Pseudomonas putida MTCC 1194 immobilized on calcium alginate

摘要

Penol in industrial wastewater has been biologically degraded by Pseudomonas putida immobilized in calcium alginate matrix. Effect of different process variables lide, initial phenol concentration, temperature, cell loading, bead size, etc. has been studied previously. An attempt has been made to explain the kinetics of the immobilized whole cell catalytic process on the basis of Michaelis-Menten (M-M) model within the phenol concentration range 100-1000 ppm. It has been observed thatat phenol concentration above 750 ppm, the reaction behavior deviate from Michaelian kinetics possible due to the effect of intraparticle diffusion. The batch experimental data have been obtained in shake flask experiments in aerobic conditions. The kinetic parameters of M-M model, v_max and K_m, and the effective diffusion coefficient (D_es) for large immobilized beads have been evaluated form Lineweaver-Burk plots and the Eadie-Hofstee plots, respectively. The simulated data satisfactorily correspond to those of the experimental values in the phenol concentraation range up to 500 ppm.