首页> 外文期刊>Developmental biology >The NMRA/NMRAL1 homologue PadA modulates the expression of extracellular cAMP relay genes during aggregation in Dictyostelium discoideum
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The NMRA/NMRAL1 homologue PadA modulates the expression of extracellular cAMP relay genes during aggregation in Dictyostelium discoideum

机译:NMRA / NMRAL1同源物PADA在Dictyostelium Discoideum中的聚集过程中调节细胞外营地继电器基因的表达

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摘要

NMRA-like proteins belong to a class of conserved transcriptional regulators that function as direct sensors of the metabolic state of the cell and link basic metabolism to changes in gene expression. PadA was the first NMRA-like protein described in Dictyostelium discoideum and was shown to be necessary for prestalk cell differentiation and correct development. We describe and characterize padA(-) mutant phenotype during the onset of development, which results in the formation of abnormally small territories and impairment of cAMP responses. Transcriptional analysis shows that cAMP-induced gene expression is downregulated in padA(-), particularly the genes that establish the extracellular cAMP relay. The mutant phenotype can be rescued with the constitutive expression of one of these genes, carA, encoding the cAMP receptor. Transcriptional analysis of padA(-)/A15::carA showed that carA maximum mRNA levels were not reached during aggregation. Our data support a regulatory role for PadA on the regulation of extracellular cAMP relay genes during aggregation and suggest that PadA is required to achieve carA full induction. (C) 2013 Elsevier Inc. All rights reserved.
机译:NMRA样蛋白属于一类保守转录调节剂,其用作细胞代谢状态的直接传感器,并将基本代谢与基因表达的变化联系起来。 PADA是Dictyostelium Discoidum中描述的第一个类似NMRA的蛋白质,并且被证明是Prestalk细胞分化和正确发育所必需的。在发育开始期间,我们描述并表征了PADA( - )突变表型,这导致形成异常小的领土和营地反应的损害。转录分析表明,CAMP诱导的基因表达在PADA( - )中下调,特别是建立细胞外营地继电器的基因。突变表型可以用这些基因中的一种,CARA,编码CAMP受体的组成型表达来拯救。 PADA( - )/ A15 :: CARA的转录分析表明,聚集期间没有达到CARA最大mRNA水平。我们的数据支持PADA在聚集期间对细胞外营地继电器基因调节的监管作用,并表明PADA需要实现CARA全诱导。 (c)2013 Elsevier Inc.保留所有权利。

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