Prevalence of white spot syndrome virus and monodon baculovirus inPenaeus monodon broodstock and postlarvae from hatcheries in southeastcoast of India

摘要

During the period March 2000 to May 2002 broodstock and postlarvae (PL) collected randomly during different months from the shrimp hatcheries in the southeast coast of India were screened for white spot syndrome virus (WSSV) and monodon baculovirus (MBV) by polymerase chain reaction (PCR). Nested PCR was performed for detection of WSSV, whereas wet-mount method and one-step PCR were carried out for MBV. Prevalence of WSSV ranged from 25 to 50% in the broodstock and 10-13% in PL by nested PCR. MBV prevalence ranged from 25 to 60% in the broodstock and 34 to 39% in the PL by non-nested PCR. A follow-up study showed that successful culture was obtained in 87% of the shrimp farms stocked with the PL that were negative for WSSV and MBV by PCR.