A self-assembled conjugated micelle with improved sensitivity for monitoring alkaline phosphatase activity

摘要

An amphiphilic coumarin derivative which forms a pi-extended micelle conformation 1 was designed and developed. The exciton efficiently migrates throughout the coumarin aggregates of 1, showing amplified fluorescence quenching in the presence of Cu2+ ions. The 1-Cu2+ complex displays a highly sensitive response to pyrophosphate (PPi), leading to 80% fluorescence recovery. The activity of alkaline phosphatase (ALP) was monitored by a real-time assay where a solution containing 1, Cu2+ and PPi in aqueous solution exhibits a sensitive turn-off fluorescence response to ALP. (C) 2019 Elsevier Ltd. All rights reserved.