One-pot doping platinum porphyrin recognition centers in Zr-based MOFs for ratiometric luminescent monitoring of nitric oxide in living cells

摘要

A new kind of nanoscale MOFs probe for nitric oxide (NO) sensing has been successfully constructed by a one-pot strategy, in which the chemically stable UiO-66 crystal structure was achieved using platinum meso-tetra(4-carboxyphenyl)porphyrin (Pt-TCPP), 1,1,2,2-Tetra(4-carboxylphenyl)ethylene (H4TCPE) and 1,4-dicarboxybenzene (BDC) as co-linkers (Pt-TCPP/H4TCPE@UiO-66). Pt-TCPP was verified to serve as a signal reporter in NO sensing fields for the first time while H4TCPE worked as a luminescence reference to build a ratiometric sensor. The integration of luminescent dyes in nanoscale MOFs effectively avoided their aggregation-caused quenching effect and poor aqueous dispersibility to rationalize NO detection in the aqueous phase. The obtained Pt-TCPP/H4TCPE@UiO-66 nanoparticles (NPs) exhibited an excellent sensing property toward NO with an ultrahigh linear correlation of the Stern-Volmer equation and a rapid response time as short as 2 min. Moreover, the elaborated sensor could work under a wide pH window (7.4, 5.6 and 0) and the limit of detection (LOD) reached as low as 0.1420 mu g mL(-1). The specificity of the obtained Pt-TCPP/H4TCPE@UiO-66 NPs toward NO sensing was scarcely affected by other possibly coexistent species in biological system. The in vitro monitoring for NO in living cells was also testified with these Pt-TCPP/H4TCPE@UiO-66 NPs.