Correlating dynamics to conformational properties: An analysis of atomic displacement parameters (B-values) in high-resolution protein structures

摘要

Atomic displacement parameters (ADPs) obtained by high resolution X-ray diffraction studies on single crystals of proteins represent the mean square displacement of atoms about their mean position. The relationship between the flexibility of the protein molecule and its conformation could be examined by a careful analysis of these ADPs. This communication presents the results of such a statistical analysis. It is shown that the ADPs are related to side chain conformations and are low for energetically favourable rotamers. Examination of the dependence of ADPs on nonplanar distortions of the peptide geometry as represented by <(omega)over bar> angle reveals that the parameters depend on the direction of non-planar distortion. Those conformations with <(omega)over bar> larger than the ideal trans geometry (180 degrees-190 degrees) are more flexible when compared to those with <(omega)over bar> < 1800 (170 degrees-180 degrees). The average ADP of a peptide unit depends weakly on the Ramachandran angles at the corresponding C alpha atom. Thus, the flexibility of different segments of the polypeptide appears to be sensitive to the conformation of the side chains as well as the main chain. [References: 17]