Enhancing the antitumor activity of tea polyphenols encapsulated in biodegradable nanogels by macromolecular self-assembly

摘要

Nanogels (NGs) with desirable stability have emerged as a promising platform for biomedical applications. Herein, a convenient approach was developed to encapsulate and protect tea polyphenols (TPs) by macromolecular self-assembly of lysozyme (Ly) and carboxymethyl cellulose (CMC) through a heating treatment. Biodegradable Ly-CMC NGs were formed on the basis of molecules driven by electrostatic interaction and hydrophobic forces. The particle size and morphology of the Ly-CMC NGs were analyzed using a Malvern particle size analyzer, fluorescence spectrophotometer, and scanning electron microscope. The results showed that the heated NGs were spherical with better stability and smaller particle size. The encapsulation efficiency of TP-loaded NGs was 89.05 +/- 3.14%, and it indicated that the Ly-CMC NGs may have a strong binding force with TPs. Moreover, TP-loaded NGs showed a sustained release feature. The DPPH and ABTS-scavenging rates of the TP-loaded NGs were 76.5% and 86.1%, respectively. The antitumor activity of the TP-loaded NGs can effectively inhibit the proliferation of HepG(2) cells. Furthermore, TP-loaded NGs were proven to significantly enhance the induction of apoptosis in hepatoma cells and exhibit obvious cell cycle arrest. Our results demonstrate that the Ly-CMC NGs have extensive application prospects as a biocompatible and biodegradable delivery carrier of food functional factors to improve their antitumor effects.