Antagonist of neuronal store-operated calcium entry exerts beneficial effects in neurons expressing PSEN1 Delta E9 mutant linked to familial Alzheimer disease

摘要

Alzheimer's disease (AD) is the neurodegenerative disorder with no cure. Recent studies suggest that dysregulated postsynaptic store-operated calcium entry (nSOCE) may underlie mushroom spine loss that is related to AD pathology. In the present study we observed that PSEN1 Delta E9 familial AD (FAD) mutation causes mushroom spine loss in hippocampal neuronal cultures. We also demonstrated that amplitude of TRPC6-mediated nSOCE is increased in PSEN1 Delta E9-expressing neurons and we suggested that inhibition of nSOCE may help to rescue synaptic defects in this model. We further established that nSOCE antagonist EVP4593 decreases PSEN1 Delta E9-mediated nSOCE upregulation and rescues mushroom spines in PSEN1 Delta E9-expressing neurons. Obtained results further highlight the connection between dysregulation of endoplasmic reticulum calcium signaling and synaptic loss in AD and suggest that calcium signaling modulators may have a therapeutic value for treatment of memory loss in AD. (C) 2019 IBRO. Published by Elsevier Ltd. All rights reserved.