Preparation of 3D-printed (Cs/PLA/PU) scaffolds modified with plasma and hybridization by Fe@PEG-CA for treatment of cardiovascular disease

摘要

One usage of tissue engineering is cells cultured on a 3D porous polymeric scaffold that is embedded in the body as tissue. The scaffolds must provide a solid primary structure for the accumulation of cells for tissue formation and be of a material that is readily absorbed by the body. The aim of the research was to prepare a series of 3D polymeric scaffolds using chitosan/polylactic acid/polyurethane (Cs/PLA/PU), modify them by plasma, bind them with gelatin on iron magnetic nanoparticles (NPs), conduct PEGylation and finally put captopril (CA) on them. Optimum conditions for scaffolding are obtained from the solution of each polymer in formic acid at 6% concentration while the best plasma application conditions are 85 s and 115 W of nitrogen plasma. The release ofin vitrosection NPs was measured at 25 degrees C with pH = 7.4. The medicine's kinetic release was studied and to examine the cytotoxicity of NPs with medicine, the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide solution (MTT) assay was generated. Antibacterial tests were also undertaken with minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC). Results indicated that the modified 3D scaffolds have greater stability and durability in the body.