The regulation of survival and differentiation of neural stem cells by miR-124 via modulating PAX3

摘要

MicroRNAs (miRNAs) have crucial functions in the regulation of proliferation and differentiation of neural stem cells (NSCs). MiR-124 has been reported to be implicated in neurogenesis. However, the precise function and mechanism of miR-124 still need further verification. In this study, we identified paired box 3 (PAX3) as a potential target of miR-124 using bioinformatics approaches. Next, we found PAX3 had reversed expression pattern with miR-124 as well as TUBB3 and GFAP. Dual-luciferase assay showed that miR-124 could bind to the 3′-UTR of PAX3 mRNA and restrain its expression. It was demonstrated that overexpression and knocking down of miR-124 in NSCs could promote the survival and suppress the apoptosis of NSCs. Meanwhile, miR-124 enhanced the expression of TUBB3 and GFAP via impairing PAX3 expression. Mechanistic study revealed that augmented Akt-GSK3β signaling pathway was the driving-force for the regulatory functions of miR-124 in NSCs. In summary, this study for the first time uncovered that miR-124 could suppress PAX3 expression, which in turn regulated the differentiation of NSCs.