Preliminary evaluation of BioFire FilmArray ? Gastrointestinal Panel for the detection of noroviruses and other enteric viruses from wastewater and shellfish

摘要

The BioFire FilmArray_(?)Gastrointestinal Panel was evaluated for the rapid detection of adenovirus, astrovirus, norovirus, rotavirus and sapovirus from influent and effluent wastewater and shellfish. The multiplex BioFire FilmArray_(?)Gastrointestinal Panel compared well to singleplex qPCR/RT-qPCR methods for the detection of adenovirus, astrovirus, rotavirus and sapovirus from influent and effluent wastewater samples. However, the BioFire FilmArray_(?)Gastrointestinal Panel?showed poor performance for the detection of norovirus, significantly underestimating its presence in wastewater and shellfish samples when compared with the singleplex?norovirus GI and GII?RT-qPCR assays. Therefore, improvement on detection efficiency for norovirus from?environmental and food samples?is necessary before using?results from?the FilmArray_(?)?Gastrointestinal Panel to assess associated?public health risks.