Preparation and characterization of C-phycocyanin peptide grafted N-succinyl chitosan by enzyme method

摘要

C phycocyanin peptide (CPC) grafted N succinyl chitosan (NSC) was prepared via the catalysis of Microbial transglutaminase (MTGase). The single factor experiment displayed that the degree of substitution (DS) of N succinyl chitosan C phycocyanin peptide (NSC CPC) depended on the reaction time, the reaction temperature and the reaction pH value. The CS, synthesized NSC and NSC CPC were characterized by Fourier transform infrared spectroscopy (FT-IR). NSC CPC showed excellent moisture absorption and retention ability. In vitro antioxidant activity assays demonstrated that, with the DS and concentration increasing of NSC CPC, the scavenging activity of 1,1 Diphenyl 2 pic rylhydrazyl (DPPH) radical and hydroxyl radical increased. The methylthiazol tetrazolium (MTT) essay demonstrated that NSC CPC inhibited Hela cells while promoted the proliferation of L929 mouse fibroblasts. In conclusion, these results suggested the potential application of NSC CPC in pharmaceutical and biomedical fields. (C) 2018 Published by Elsevier B.V.