Titanium dioxide nanotubes incorporated gellan gum bio-nanocomposite film for wound healing: Effect of TiO2 nanotubes concentration

摘要

The synthesized titanium dioxide nanotubes (TiO2-NTs) were emerged as wound healing enhancer as well as exhibited significant wound healing activity on Sprague Dawley rats. In our present study, the blends of GG and TiO2-NTs bio-nanocomposite film was characterised by fourier transform infrared (FTIR), x-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis, atomic force microscopy (AFM). The morphology of TiO2-NTs was investigated using transmission electron microscopy (TEM). The mechanical properties study shows that the GG + TiO2-NTs (20 w/w %) bionanocomposite film possessed the highest tensile strength and young modulus which are (4.56 +/- 0.15) MPa and (68 +/- 1.63) MPa, respectively. GG + TiO2-NTs (20 w/w %) also displays the highest antibacterial activity with (16 +/- 0.06) mm, (16 +/- 0.06) mm, (14 +/- 0.06) mm, and (12 +/- 0.25) mm inhibition zone were recorded against Staphylococcus aureus, Streptococcus, Escherichia coli, and Pseudomonas aeruginosa. The prepared bio-nanocomposite films have good biocompatibility against 3T3 mouse fibroblast cells and caused accelerated healing of open excision type wounds on Sprague Dawley rat model. The synergistic effects of bio-nanocomposite film like good swelling and WVTR properties, excellent hydrophilic nature, biocompatibility, wound appearance and wound closure rate through in vivo test makes it a suitable candidate for wound healing applications. (C) 2019 Elsevier B.V. All rights reserved.