Novel Schiff Base of E-2-(((4-Aminophenyl)imino)methyI)-5-(difluoromethoxy)phenol Fluorescence Chemosensor for Detection of Al~(3+), Fe~(2+), Cu~(2+) Ions and its Application towards Live Cell Imaging

摘要

A Schiff base compound E-2-(((4-aminophenyl)imino)mefhyl)-5-(difluoromethoxy)phenol was synthesized and characterized by FT-IR, ~1H and ~(13)C NMR, ESI-mass spectroscopy. The synthesized compound also selectively detects Al~(3+), Fe~(2+) and Cu~(2+) without any interference of other metal ions. Fluorescence titrations carried out to find the selectivity of Al~(3+), Fe~(2+) and Cu~(2+) in turn-on system, with binding modes of 2:1 complex, confirmed by Job's plot. The presence of metal ions Al~(3+), Fe~(2+) and Cu~(2+) with receptor conformed by ESI-MS spectrum, which changed the base value at 298.00 m/z. Moreover, among the binding constant of three metals calculated (20 μM), Al~(3+) showed a high value of 5.7 × 10~4 M~(-1) compared to Fe~(2+) and Cu~(2+) metal ions. Prominently, the cytotoxicity activities of probe with HeLa cells were also calculated.