The Tail That Wags the Dog: Topoisomerase IV ParC C-Terminal Domain Controls Strand Passage Activity through Multipartite Topology-Dependent Interactions with DNA

摘要

Type HA topoisomerases are essential enzymes that contribute to chromosomal integrity by controlling the degree of supercoiling and segregating newly replicated chromosomes [1]. They share a conserved core mechanism in which a double-stranded segment of DNA is passed through a transient double-stranded break in a second segment of DNA (Fig. 1). Remarkably, this conserved strand passage reaction results in a range of distinct activities catalyzed by different but related type HA topoisomerases [2]. At one extreme is prokaryotic DNA gyrase that negatively supercoils DNA but is a poor decatenase.