Enzyme-Electropolymer-Based Amperometric Biosensors:An Innovative Platform for Time-Temperature Integrators

摘要

A novel exogenous time-temperature integrator(TTI)based on an amperometric glucose oxidase biosensor is presented.The TTI consists of the enzyme entrapped within an electrochemically generated poly(ophenylenediamine)(PoPD)thin film deposited on the interior wall of a platinum(Pt)or a platinized stainless steel(Pt-SS)capsule.After thermal treatment,the TTI is mounted in a continuous flow system and connected to a potentiostat for amperometric detection of residual enzyme activity.A measurement is completed within 10 min.Isothermal treatments were carried out between 70 and 79.7 deg C.Thermal inactivation of the immobilized enzyme followed apparent first-order kinetics with z values of 6.2 +-0.6 and 6.6 +-0.8 deg C for Pt and Pt-SS capsules,respectively.These z values suggest that the proposed TTIs have the potential to assess pasteurization processes that target microorganism such as Listeria monocytogenes and Escherichia coli O157:H7.