Ultra-rapid freezing and storage of rat embryos in an electric refrigerator at-130 degrees C without liquid cryo-agents, with ultra-short exposure in the freezing medium and direct rehydration after thawing

摘要

An ultra-rapid freezing technique for embryos has been developed. This procedure involves: ultra-short exposure to cryoprotectants; freezing of embryos in a metallic powder pre-cooled in an electric refrigerator at -130 degrees C; storage of embryos in a refrigerator at -130 degrees C; direct rehydration after thawing. [References: 32]