Comment on 'killer Ig-like Receptor 2DL4 Does Not Mediate NK Cell IFN-γ Responses to Soluble HLA-G Preparations'

摘要

We read with interest the article by Le Page et al. (4), who did not find evidence of functional interaction between soluble HLA-G and KIR2DL4, and question the validity of earlier reports. The conclusion that dieir findings "raise serious doubts about many published results suggesting that IFN-7 production by NK cells can be directly stimulated through KIR2DL4 by sHLA-G or soluble Abs" is premature, given that their work differed from other studies in several important ways: 1) The response of NK cells to soluble HLA-G, as measured by IFN-7, was dependent on contaminating DCs in NK cells purified using the Rosette Sep technique (Stem Cell Technologies). More highly purified NK cells using the EasySep method, (Stem Cell Technologies) did not respond (4).