首页> 外文期刊>Plant physiology >Mapping of a Cellulose-Deficient Mutant Named dwarf1-1 in Sorghum bicolor to the Green Revolution Gene gibberellin20-oxidase Reveals a Positive Regulatory Association between Gibberellin and Cellulose Biosynthesis
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Mapping of a Cellulose-Deficient Mutant Named dwarf1-1 in Sorghum bicolor to the Green Revolution Gene gibberellin20-oxidase Reveals a Positive Regulatory Association between Gibberellin and Cellulose Biosynthesis

机译:高粱双色中缺乏纤维素的突变体dwarf1-1与绿色革命基因gibberellin20-氧化酶的映射揭示了赤霉素和纤维素生物合成之间的正调控关联。

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摘要

Here, we show a mechanism for expansion regulation through mutations in the green revolution gene gibberellin20 (GA20)-oxidase and show that GAs control biosynthesis of the plants main structural polymer cellulose. Within a 12,000 mutagenized Sorghum bicolor plant population, we identified a single cellulose-deficient and male gametophyte-dysfunctional mutant named dwarf1-1 (dwf1-1). Through the Sorghum propinquum male/dwf1-1 female F2 population, we mapped dwf1-1 to a frameshift in GA20-oxidase. Assessment of GAs in dwf1-1 revealed ablation of GA. GA ablation was antagonistic to the expression of three specific cellulose synthase genes resulting in cellulose deficiency and growth dwarfism, which were complemented by exogenous bioactive gibberellic acid application. Using quantitative polymerase chain reaction, we found that GA was positively regulating the expression of a subset of specific cellulose synthase genes. To cross reference data from our mapped Sorghum sp. allele with another monocotyledonous plant, a series of rice (Oryza sativa) mutants involved in GA biosynthesis and signaling were isolated, and these too displayed cellulose deficit. Taken together, data support a model whereby suppressed expansion in green revolution GA genes involves regulation of cellulose biosynthesis.
机译:在这里,我们展示了通过绿色革命基因gibberellin20(GA20)-氧化酶中的突变进行扩展调控的机制,并表明GAs控制着植物主要结构聚合物纤维素的生物合成。在12,000个诱变的高粱双色植物种群中,我们鉴定了一个名为dwarf1-1(dwf1-1)的单一纤维素缺乏和雄配子体功能障碍的突变体。通过高粱原虫男性/ dwf1-1女性F2种群,我们将dwf1-1映射到GA20氧化酶的移码。 dwf1-1中的GA评估显示GA消融。 GA消融拮抗导致纤维素缺乏和生长障碍的三种特定纤维素合酶基因的表达,而外源生物活性赤霉酸的应用可以弥补这些缺陷。使用定量聚合酶链反应,我们发现GA正调节特定纤维素合成酶基因的一个子集的表达。从我们映射的高粱sp。等位基因与另一单子叶植物,分离了一系列与GA生物合成和信号传导有关的水稻(Oryza sativa)突变体,这些突变体也显示出纤维素缺乏。综上所述,数据支持一个模型,其中绿色革命GA基因的抑制扩增涉及纤维素生物合成的调控。

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