EF-Tu dynamics during pre-translocation complex formation: EF-Tu center dot GDP exits the ribosome via two different pathways

摘要

The G-protein EF-Tu, which undergoes a major conformational change when EF-Tu center dot GTP is converted to EF-Tu center dot GTP, forms part of an aminoacyl(aa)-tRNA center dot EF-Tu center dot GTP ternary complex (TC) that accelerates the binding of aa-tRNA to the ribosome during peptide elongation. Such binding, placing a portion of EF-Tu in contact with the GTPase Associated Center (GAC), is followed by GTP hydrolysis and Pi release, and results in formation of a pretranslocation (PRE) complex. Although tRNA movement through the ribosome during PRE complex formation has been extensively studied, comparatively little is known about the dynamics of EF-Tu interaction with either the ribosome or aa-tRNA. Here we examine these dynamics, utilizing ensemble and single molecule assays employing fluorescent labeled derivatives of EF-Tu, tRNA, and the ribosome to measure changes in either FRET efficiency or fluorescence intensity during PRE complex formation. Our results indicate that ribosome-bound EF-Tu separates from the GAC prior to its full separation from aa-tRNA, and suggest that EF-Tu center dot GDP dissociates from the ribosome by two different pathways. These pathways correspond to either reversible EF-Tu center dot GTP dissociation from the ribosome prior to the major conformational change in EF-Tu that follows GTP hydrolysis, or irreversible dissociation after or concomitant with this conformational change.