The chemoanatomical organization of the visual sector of the cat's thalamic reticular nucleus (TRN)-that is at the dorsal lateral geniculate nucleus (dLGN) and at the pulvinar nucleus (Pul)-was investigated with two novel cytoarchitectonic markers. The Wisteria floribunda agglutinin (WFA) binding reaction visualized the extracellular perineuronal net (PN) and the SMI 32 immunoreaction stained intracellular neurofilaments. Two distinct layers of the TRN could be detected, particularly by WFA- but also by SMI 32-staining. The outer tier outlined a canopy of labeling placed a bit detached from the diencephalon dorsolaterally, while the inner TRN tier is very tightly attached to the thalamic lamina limitans externa. The labeled neurons showed typically fusiform morphology with dendrites orienting in the plane of TRN. Additionally, these chemoarchitectural reactions identified a chain of structures in the ventral diencephalon connected to the TRN tiers. One stained string is formed by the subthalamic nucleus bound laterally to the peripeduncular nucleus extending further dorsolateral into the outer TRN tier. The other chain laced up the field of Forel, the zona incerta, the ventral LGN, the perigeniculate nucleus (PGN) and the previously-overlooked peripulvinar nucleus (PPulN) and so formed the inner TRN tier. In the third most distanced TRN tier, in the perireticular nucleus, a very few WFA-binding presenting neuron were found. In addition to the PN possessing TRN neurons, WFA-reactive presumable interneurons were also labeled within the visual thalamus. Following tracer injections into the feline Pul, two stripes of cells were retrogradely labeled in the neighboring visual TRN sector. The location of these reticular neurons coincided precisely with the chemoanatomically identified inner and outer TRN tiers. On the analogy of the PGN-TRN duality at the dLGN, the chemoanatomical and tract tracing findings strongly suggest a similar dual organization in the pulvinoprojecting TRN portion.
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