Expression of cystic fibrosis transmembrane conductance regulator in ganglion cells of the hearts.

摘要

Cystic fibrosis transmembrane conductance regulator (CFTR) as an important chloride-selective channel is known to distribute on the apical membrane of chloride-secreting epithelial cells. However, CFTR is also reported to express in the neurons of human and rat brain. In this study we aim to investigate the expression of CFTR in ganglion cells of the hearts. We used immunohistochemistry, in situ hybridization, laser microdissection (LMD) and nested reverse transcriptase polymerase chain reaction (nested RT-PCR) to detect CFTR in the ganglion cells of the Sprague-Dawley rat hearts and found widespread and abundant the expression of CFTR protein and its mRNA in the ganglion cells of the rat hearts. The presence of CFTR in ganglia does not only provide a possible explanation for cardiovascular symptoms of cystic fibrosis patients but also may lead to a better understanding of a possible role for CFTR in the neuronal regulation of the heart.