Expression of m-Golsyn/Syntabulin gene during mouse brain development.

摘要

We recently isolated the cDNA for the mouse Golsyn/Syntabulin (m-Golsyn/Syntabulin) gene and mapped it to mouse chromosome 15B3.2 syntenic with human chromosome 8q23, on which a locus responsible for primary open-angle glaucoma had been located. In the present study, we examined the expression of m-Golsyn/Syntabulin protein in various regions of mouse brain and its developmental changes by use of anti-GOLSYN antibody. m-Golsyn/Syntabulin protein was detected in various brain regions at embryonic day 14 and throughout the postnatal stages. Furthermore, as the histogenesis and maturation of brain proceeded, strong expression of the protein became detectable in cells of the choroid plexus, piriform cortex, pyramidal cell layer, and Purkinje cell layer. In situ hybridization analysis of the mouse brain revealed that localization of the m-Golsyn/Syntabulin transcript was very similar to that of m-Golsyn/Syntabulin protein, confirming the high-level expression of the m-Golsyn/Syntabulin gene in the specific brain regions. High-level expression of m-Golsyn/Syntabulin protein was also observed in the ocular tissues including the ciliary body, which is known as a site for the production of aqueous humor. These results may indicate a significant role for this protein in neuronal cells and other types of cells such as those of the choroid plexus and ciliary body.