Nitric oxide synthase/K+ channel cascade triggers the adenosine A2B receptor-sensitive renal vasodilation in female rats

摘要

Adenosine A2B-receptors mediate the adenosine-evoked renal vasodilations in male rats. Here, we tested whether this finding could be replicated in female renal vasculature and whether K+ hyperpolarization induced by nitric oxide synthase (NOS) and/or heme oxygenase (HO) accounts for adenosine A2B receptor-sensitive renal vasodilations. In phenylephrine-preconstricted perfused kidneys, vasodilations caused by the adenosine analog 5??-N-ethylcarboxamidoadenosine (NECA, 1.6-50 nmol) were attenuated after blockade of adenosine A2B (alloxazine) but not A2A [8-(3-Chlorostyryl) caffeine, CSC] or A 3 receptors (N-(2-methoxyphenyl)-N??-[2-(3-pyridinyl)-4- quinazolinyl]-urea, VUF 5574), confirming the preferential involvement of A 2B receptors in NECA responses. NOS activation mediated the A 2B receptor-mediated NECA response because: (i) NOS inhibition (N??-nitro-l-arginine-methyl ester, l-NAME) attenuated NECA vasodilations, (ii) concurrent l-NAME/alloxazine exposure caused more inhibition of NECA responses, and (iii) inhibition of NECA responses by alloxazine disappeared in l-arginine-supplemented preparations. Although HO inhibition (zinc protoporphyrin) failed to modify NECA responses, the attenuation of these responses by alloxazine disappeared in hemin (HO inducer)-treated preparations. NECA vasodilations were also attenuated after exposure to BaCl2, glibenclamide but not tetraethylammonium (blockers of inward rectifier, ATP-sensitive, and Ca2+-dependent K+-channels, respectively). The combined alloxazine/BaCl2/glibenclamide infusion caused no additional attenuation of NECA vasodilations. Vasodilations caused by minoxidil (K+-channel opener) were reduced by l-NAME or BaCl 2/glibenclamide, supporting the importance of NOS signaling in K + hyperpolarization. NECA or minoxidil vasodilations were attenuated by ouabain, Na+/K+-ATPase inhibitor, and in KCl-preconstricted preparations. Overall, facilitation of adenosine A 2B receptor/NOS/K+ channel/Na+/K +-ATPase cascade underlies NECA vasodilations in female rats. Enhancing HO activity, albeit not causally related to NECA vasodilations, improves the pharmacologically compromised (alloxazine) NECA response. ? 2013 Elsevier B.V.