Evaluation of Phenolic Compounds and Development of Chromatographic Profiles in Spathodea campanulata Inflorescence by HPTLC

摘要

In the present work, an investigation of phenolic constituents in flower petals of Spathodea campanulata was carried out. Ethyl alcohol, 50 % v/v ethyl alcohol and distilled water were used as solvents for the optimization of extraction process. The extracts obtained were subjected to phytochemical screening and quantified for total phenolic content and total flavonoid content. Total phenolic content of the extracts obtained were in the sequence: distilled water (25.75) >50 % ethyl alcohol (15.12) >ethyl alcohol (2.37), which was expressed in terms of mg/g gallic acid equivalent (GAE) of sample. Whereas total flavonoid content of the extracts were in the sequence: distilled water (17.65) >50 % ethyl alcohol (10.25) >ethyl alcohol (2.90) which was expressed in terms of mg/g rutin equivalent (RE) of sample. High performance thin layer chromatography analysis of the sample extracts was performed to obtain chromatographic profiles, scanning was performed at 270, 320 and 370 nm and the R_f values obtained for the sample extracts were compared with the R_f values of the flavonoid compounds viz., rutin, catechin and quercetin.