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Dual-grating confocal-rainbow volume holographic imaging system designs for high depth resolution

机译:用于高深度分辨率的双光栅共聚焦彩虹体全息成像系统设计

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Confocal microscopy rejects out-of-focus light from the object by scanning a pinhole through the image and reconstructing the image point by point. Volume holographic imaging systems with bright-field illumination have been proposed as an alternative to conventional confocal-type microscopes that does not require scanning of a pinhole or a slit. However, due to wavelength-position degeneracy of the hologram, the high Bragg selectivity of the volume hologram is not utilized and system performance is not optimized. Confocal-rainbow illumination has been proposed as a means to remove the degeneracy and improve optical sectioning in these systems. In prior work, two versions of this system were illustrated: the first version had a separate illumination and imaging grating and the second used a single grating to disperse the incident light and to separate wavelengths in the imaging path. The initial illustration of the dual-grating system has limited depth resolution due to the low selectivity of the illumination grating. The initial illustration of the single-grating system has high depth resolution but does not allow optimization of the illumination path and requires high optical quality of the holographic filters. In this paper we consider the design and tolerance requirements of the dual-grating system for high depth resolution and demonstrate the results with an experimental system. An experimental system with two 1.8 mm thick planar holograms achieved a depth resolution of 7 μm with a field of view of 1.9 mm and a hologram dispersion matching tolerance of ±0.008°.
机译:共聚焦显微镜通过扫描穿过图像的针孔并逐点重建图像,从而排除了来自对象的散焦光。已经提出了具有明场照明的体积全息成像系统,作为不需要扫描针孔或狭缝的常规共焦型显微镜的替代品。但是,由于全息图的波长位置简并性,所以没有利用体积全息图的高布拉格选择性,并且系统性能没有得到优化。共焦彩虹照明已被提出作为消除简并并改善这些系统中光学截面的一种手段。在先前的工作中,说明了该系统的两个版本:第一个版本具有单独的照明和成像光栅,第二个版本使用单个光栅来分散入射光并分离成像路径中的波长。由于光栅的低选择性,双光栅系统的初始图示具有有限的深度分辨率。单光栅系统的初始图示具有较高的深度分辨率,但不允许优化照明路径,并且需要全息滤光镜的高光学质量。在本文中,我们考虑了双光栅系统对高深度分辨率的设计和公差要求,并通过实验系统演示了结果。具有两个1.8 mm厚的平面全息图的实验系统实现了7μm的深度分辨率,1.9 mm的视场和±0.008°的全息图色散匹配公差。

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