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Afterpulsing and its correction in fluorescence correlation spectroscopy experiments

机译:荧光相关光谱实验中的后脉冲及其校正

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摘要

Afterpulsing arises from feedback in a photon detector. This means that each real signal pulse can be followed by an afterpulse at a later time. This effect is particularly troubling in photon correlation experiments. Few treatments of this effect have appeared in the literature, and few software programs to solve the problem have been written. We demonstrate the afterpulsing effect in fluorescence correlation spectroscopy by using different avalanche photodiodes. We prove theoretically that under simple and reasonable conditions afterpulsing in autocorrelation can be eliminated to the leading order; we have found it easy to program software for the correction. We compare our results with those from cross correlation. We also discuss some experimental parameters that may affect the afterpulsing.
机译:后脉冲由光子检测器中的反馈引起。这意味着每个真实信号脉冲之后都可以跟随一个后脉冲。在光子相关实验中,这种影响尤其令人不安。文献中几乎没有这种效果的治疗方法,解决该问题的软件程序也很少。我们通过使用不同的雪崩光电二极管展示了荧光相关光谱中的后脉冲效应。我们从理论上证明,在简单合理的条件下,自相关的后脉冲可以消除到前导阶。我们发现很容易对软件进行编程以进行更正。我们将我们的结果与互相关的结果进行比较。我们还将讨论一些可能会影响后脉冲的实验参数。

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    《Applied optics》 |2003年第19期|共6页
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