首页> 外文期刊>Analytical chemistry >Utilizing a Key Aptamer Structure-Switching Mechanism for the Ultrahigh Frequency Detection of Cocaine
【24h】

Utilizing a Key Aptamer Structure-Switching Mechanism for the Ultrahigh Frequency Detection of Cocaine

机译:利用关键适体结构转换机制进行可卡因超高频检测

获取原文
获取原文并翻译 | 示例
       

摘要

Aptasensing of small molecules remains a challenge as detection often requires the use of labels or signal amplification methodologies, resulting In both difficult-to-prepare sensor platforms and multistep, complex assays. Furthermore, many aptasensors rely on the binding mechanism or structural changes associated with target capture by the aptameric probe, resulting in a detection scheme customized to each aptamer. It is in this context that we report herein a sensitive cocaine aptasensor that offers both real-time and label-free measurement capabilities. Detection relies on the electromagnetic piezoelectric acoustic sensor (EMPAS) platform. The sensing interface consists of a S-(11-trichlorosilyl-undecanyl)benzenethiosulfonate (BTS) adlayer-coated quartz disc onto which a structure-switching cocaine aptamer (MN6) is immobilized, completing the preparation of the MN6 cocaine aptasensor (M6CA). The EMPAS system has recently been employed as the foundation of a cocaine aptasensor based on a structurally rigid cocaine aptamer variant (MN4), an aptasensor referred to by analogy as M4CA. M6CA represents a significant increase in terms of analytical performance, compared to not only M4CA but also other cocaine aptamer-based sensors that do not rely on signal amplification, producing an apparent K-d of 27 +/- 6 mu M and a 0.3 mu M detection limit. Remarkably, the latter is in the range of that achieved by cocaine aptasensors relying on signal amplification. Furthermore, M6CA proved to be capable not only of regaining its cocaine-binding ability via simple buffer flow over the sensing interface (i.e., Without the necessity to implement an additional regeneration step, such as in the case of M4CA), but also of detecting cocaine in a multicomponent matrix possessing potentially assay-interfering species. Finally, through observation of the distinct shape of its response profiles to cocaine injection, demonstration was made that the EMPAS system in practice offers the possibility to distinguish between the binding mechanisms of structure-switching (MN6) vs rigid (MN4) aptameric probes, an ability that could allow the EMPAS to provide a more universal aptasensing platform than what is ordinarily observed in the literature.
机译:小分子的感知仍然是一个挑战,因为检测通常需要使用标记或信号放大方法,从而导致难以准备的传感器平台和多步,复杂的测定。此外,许多适体传感器依赖于与适体探针捕获靶标相关的结合机制或结构变化,从而导致为每种适体定制的检测方案。在此背景下,我们在此报告了一种可卡因适体传感器,该传感器可提供实时和无标签的测量功能。检测依赖于电磁压电声学传感器(EMPAS)平台。传感界面由S-(11-三氯甲硅烷基-十一碳二烯基)苯硫代磺酸盐(BTS)涂有涂层的石英圆盘组成,上面固定了结构转换可卡因适体(MN6),从而完成了MN6可卡因适体传感器(M6CA)的制备。 EMPAS系统最近已被用作基于结构刚性可卡因适体变体(MN4)的可卡因适体传感器的基础,该可卡因适体变体类似地称为M4CA。与M4CA相比,M6CA代表了分析性能的显着提高,而且与其他不依赖信号放大的可卡因适体传感器相比,其表观Kd为27 +/- 6μM,检测灵敏度为0.3μM限制。值得注意的是,后者在依靠信号放大的可卡因适体传感器所能达到的范围内。此外,事实证明,M6CA不仅能够通过传感接口上的简单缓冲液流来恢复其可卡因结合能力(即,无需执行额外的再生步骤,例如在M4CA的情况下),而且还能够检测可卡因在多成分基质中具有潜在的干扰测定的物种。最后,通过观察其对可卡因注射反应曲线的独特形状,证明了EMPAS系统在实践中提供了区分结构转换(MN6)与刚性(MN4)适体探针的结合机制的可能性,能够使EMPAS提供比文献中通常观察到的更通用的适体平台的能力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号