Dual-Color Imaging of Magnesium/Calcium Ion Activities with Two-Photon Fluorescent Probes

摘要

We report two-photon probes (FMg1 and FMg2) that can selectively detect intracellular free Mg~(2+) ([Mg~(2+)]_i) in live cells and tissues by two-photon microscopy. Combined with BCaM, a two-photon probe for near-membrane Ca~(2+) ([Ca~(2+)]_m), FMg2 allows dual-color imaging of Mg~(2+)/Ca~(2+) activities in live cells and [Mg~(2+)]_i /[Ca~(2+)]_m distributions in live tissues at a depth of 100-200 μm.