Ultratrace Enrichment of Tyrosine Phosphorylated Peptides on an Imprinted Polymer

摘要

Novel molecularly imprinted polymers (MIPs) designed to bind the side chain ofphosphotyrosine can be used as artificial receptors for affinity-based enrichment of proteolytic peptides. In comparison with general enrichment methods for phosphorylated peptides such as TiO_(2)-based methods, the pTyr-imprinted polymers offered high selectivity for pTyr-containing peptides down to the low fmol level. This suggests MIPs as a new tool for affinity-based proteomics.