A Designed Functional Metalloenzyme that Reduces O2 to H2O with Over One Thousand Turnovers

摘要

Much progress has been made in designing metalloproteins with structures similar to native enzymes and advances in computational biology have allowed for rational design of enzyme function as well. Despite these achievements, most designed enzymes have simple active site structures and low activities, with limited turnover numbers. Designing artificial enzymes with higher complexity and number of turnovers is not only an important measure of success in the field, but can also reveal the structural features responsible for tuning enzymatic activities and may result in artificial enzymes for practical applications.